Human IL-7 R alpha&TSLPR Protein (ILT-HM107)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-7R alpha&TSLPR, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TSLP, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). The affinity constant of 15.54 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Glu21-Asp239(IL-7 R alpha) & Gln23-Lys231(TSLPR)

Catalog: ILT-HM107

$345.00
Size:

Order now get it on

Product Description Recombinant Human IL-7 R alpha&TSLPR Protein is expressed from HEK293 with His tag at the C-terminus.It contains Glu21-Asp239 (IL-7 R alpha) and Gln23-Lys231 (TSLPR).

Product Background

IL-7R alpha & TSLPR is an heterodimeric receptor, with TSLP as the ligand, which initiates downstream signaling pathways upon binding to TSLP and subsequently participates in thymus development and immune cell generation. Among them, IL-7R alpha is a component of both the IL-7 and TSLP receptors.

Product Category

Recombinant Protein

Target

IL-7 R alpha&TSLPR
Accession

Species

Human

Amino Acid Range

Glu21-Asp239(IL-7 R alpha) & Gln23-Lys231(TSLPR)
Molecular Weight The protein has a predicted MW of 31.18 kDa (IL-7 R alpha) and 29.03 kDa (TSLPR). Due to glycosylation, the protein migrates to 58-68 kDa (IL-7 R alpha) and 50-58 kDa (TSLPR) based on Tris-Bis PAGE result.

Activity

Immobilized Human IL-7R alpha&TSLPR, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TSLP, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). The affinity constant of 15.54 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human IL-7R alpha&TSLPR, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-7, No Tag with an affinity constant of 15.54 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-7R alpha&TSLPR, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TSLP, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).
The purity of Human IL-7 R alpha&TSLPR is greater than 95% as determined by SEC-HPLC.
Human IL-7 R alpha&TSLPR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

You may also be interested in