- Express System: HEK293
- Product Tag: C-His
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Human IL-7R alpha&TSLPR, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TSLP, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). The affinity constant of 15.54 nM as determined in SPR assay (Biacore T200). See testing image for detail.
- Amino Acid Range: Glu21-Asp239(IL-7 R alpha) & Gln23-Lys231(TSLPR)
Product Description |
Recombinant Human IL-7 R alpha&TSLPR Protein is expressed from HEK293 with His tag at the C-terminus.It contains Glu21-Asp239 (IL-7 R alpha) and Gln23-Lys231 (TSLPR). |
Product Background |
IL-7R alpha & TSLPR is an heterodimeric receptor, with TSLP as the ligand, which initiates downstream signaling pathways upon binding to TSLP and subsequently participates in thymus development and immune cell generation. Among them, IL-7R alpha is a component of both the IL-7 and TSLP receptors. |
Product Category |
Recombinant Protein |
Target |
IL-7 R alpha&TSLPR |
Synonyms |
IL7Ra; CD127; TSLP R; CRLF2; IL-XR; ILXR; p2RY8; TSLPR; CRL2; CRLF2Y |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu21-Asp239(IL-7 R alpha) & Gln23-Lys231(TSLPR) |
Molecular Weight |
The protein has a predicted MW of 31.18 kDa (IL-7 R alpha) and 29.03 kDa (TSLPR). Due to glycosylation, the protein migrates to 58-68 kDa (IL-7 R alpha) and 50-58 kDa (TSLPR) based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-7R alpha&TSLPR, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TSLP, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). The affinity constant of 15.54 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in PBS (pH 7.4). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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