• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 90% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-7R alpha&TSLPR, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TSLP, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). The affinity constant of 15.54 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Glu21-Asp239(IL-7 R alpha) & Gln23-Lys231(TSLPR)
Catalog #ILT-HM107
Price
Regular price $379.50
Regular price Sale price $379.50
/
Size
Avantor VWR Fisher Scientific

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Human IL-7 R alpha&TSLPR Protein (ILT-HM107) Summary

Product Description

 Recombinant Human IL-7 R alpha&TSLPR Protein is expressed from HEK293 with His tag at the C-terminus of IL-7 R alpha.It contains Glu21-Asp239 (IL-7 R alpha) and Gln23-Lys231 (TSLPR).

Product Background

 IL-7R alpha & TSLPR is an heterodimeric receptor, with TSLP as the ligand, which initiates downstream signaling pathways upon binding to TSLP and subsequently participates in thymus development and immune cell generation. Among them, IL-7R alpha is a component of both the IL-7 and TSLP receptors.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human IL-7 R alpha&TSLPR Protein (ILT-HM107) Specifications/Details

Protein
 IL-7 R alpha&TSLPR
Synonyms
 IL7Ra; CD127; TSLP R; CRLF2; IL-XR; ILXR; p2RY8; TSLPR; CRL2; CRLF2Y
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Glu21-Asp239(IL-7 R alpha) & Gln23-Lys231(TSLPR)
Molecular Weight
 The protein has a predicted MW of 31.18 kDa (IL-7 R alpha) and 29.03 kDa (TSLPR). Due to glycosylation, the protein migrates to 58-68 kDa (IL-7 R alpha) and 50-58 kDa (TSLPR) based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-7R alpha&TSLPR, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TSLP, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). The affinity constant of 15.54 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 90% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human IL-7R alpha&TSLPR, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-7, No Tag with an affinity constant of 15.54 nM as determined in SPR assay (Biacore T200). Human IL-7R alpha&TSLPR, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-7, No Tag with an affinity constant of 15.54 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-7R alpha&TSLPR, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TSLP, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). Immobilized Human IL-7R alpha&TSLPR, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TSLP, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).
Human IL-7 R alpha&TSLPR on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. Human IL-7 R alpha&TSLPR on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Loaded Human TSLP, Fc Tag on ProA-Biosensor can bind IL-7 R alpha&TSLP R, His Tag with an affinity constant of 0.37 nM as determined in BLI assay . Loaded Human TSLP, Fc Tag on ProA-Biosensor can bind IL-7 R alpha&TSLP R, His Tag with an affinity constant of 0.37 nM as determined in BLI assay .
The purity of Human IL-7 R alpha&TSLPR is greater than 95% as determined by SEC-HPLC. The purity of Human IL-7 R alpha&TSLPR is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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