Human IL-7R alpha/CD127 Protein (IL7-HM2RA)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-7 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 18.1ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Glu21-Gly236

Catalog: IL7-HM2RA

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Product Description Recombinant Human IL-7R alpha/CD127 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu21-Gly236.

Product Background

Interleukin 7 (IL-7) and its receptor (IL-7R, a heterodimer of IL-7Rα and γc) are essential for normal lymphoid development. IL-7 and IL-7R activate three main pathways: STAT5, PI3K/Akt/mTOR and MEK/Erk, ultimately leading to the promotion of leukemia cell viability, cell cycle progression and growth.

Product Category

Recombinant Protein

Target

IL-7R alpha/CD127
Synonyms IL-17R alpha; IL7Ra; CD127; CDW127; ILRA

Gene/Protein ID

P16871-1

Species

Human

Exact Sequence

Glu21-Gly236
Molecular Weight The protein has a predicted MW of 51.6 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human IL-7 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 18.1ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-7 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 18.1ng/ml determined by ELISA.
The purity of Human IL-7R alpha is greater than 95% as determined by SEC-HPLC.
Human IL-7R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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