Human IL-7R alpha/CD127 Protein (IL7-HM2RA)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-7 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 52.5ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Glu21-Gly236

Catalog: IL7-HM2RA

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Product Description	Recombinant Human IL-7R alpha/CD127 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu21-Gly236.
Product Background	Interleukin 7 (IL-7) and its receptor (IL-7R, a heterodimer of IL-7Rα and γc) are essential for normal lymphoid development. IL-7 and IL-7R activate three main pathways: STAT5, PI3K/Akt/mTOR and MEK/Erk, ultimately leading to the promotion of leukemia cell viability, cell cycle progression and growth.
Product Category	Recombinant Protein

Target	IL-7R alpha/CD127
Synonyms	IL-17R alpha; IL7Ra; CD127; CDW127; ILRA
Gene/Protein ID	P16871-1
Species	Human
Exact Sequence	Glu21-Gly236
Molecular Weight	The protein has a predicted MW of 51.6 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human IL-7 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 52.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human IL-7 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 52.5ng/ml determined by ELISA.
	The purity of Human IL-7R alpha is greater than 95% as determined by SEC-HPLC.
	Human IL-7R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.