Human IL-8/CXCL8 Protein (IL8-HM1RA) Summary
Product Description |
Recombinant Human IL-8/CXCL8 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser28-Ser99. |
Product Background |
Interleukin-8 (IL-8) has been revealed as a critical regulator of CNS function and development with participation in many CNS disorders including gliomas.Several promising approaches that target directly or indirectly IL-8 effects in gliomas are currently in progress while more-in-depth studies are needed to validate its biomarker role and elucidate the underlying molecular mechanisms. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human IL-8/CXCL8 Protein (IL8-HM1RA) Specifications/Details
Protein |
IL-8/CXCL8 |
Synonyms |
Interleukin-8; IL-8; GCP-1; MDNCF; MONAP; NAP-1; GCP/IL-8 protein IV; IL8/NAP1 form I; CXCL8; LECT; LUCT; LYNAP; NAF |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser28-Ser99 |
Molecular Weight |
The protein has a predicted MW of 9.5 kDa. Due to glycosylation, the protein migrates to 11-13 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-8, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-8 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Human IL-8, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-8 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. |
|
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Human IL-8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human IL-8 is greater than 95% as determined by SEC-HPLC. |
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