Human IL-8/CXCL8 Protein (IL8-HM1RA)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-8, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-8 Antibody, hFc Tag with the EC50 of 12.5ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ser28-Ser99

Catalog: IL8-HM1RA

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Product Description	Recombinant Human IL-8/CXCL8 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser28-Ser99.
Product Background	Interleukin-8 (IL-8) has been revealed as a critical regulator of CNS function and development with participation in many CNS disorders including gliomas.Several promising approaches that target directly or indirectly IL-8 effects in gliomas are currently in progress while more-in-depth studies are needed to validate its biomarker role and elucidate the underlying molecular mechanisms.
Product Category	Recombinant Protein

Target	IL-8/CXCL8
Synonyms	Interleukin-8; IL-8; GCP-1; MDNCF; MONAP; NAP-1; GCP/IL-8 protein IV; IL8/NAP1 form I; CXCL8; LECT; LUCT; LYNAP; NAF
Gene/Protein ID	P10145-1
Species	Human
Exact Sequence	Ser28-Ser99
Molecular Weight	The protein has a predicted MW of 9.5 kDa. Due to glycosylation, the protein migrates to 11-13 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human IL-8, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-8 Antibody, hFc Tag with the EC50 of 12.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human IL-8, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-8 Antibody, hFc Tag with the EC50 of 12.5ng/ml determined by ELISA.
	The purity of Human IL-8 is greater than 95% as determined by SEC-HPLC.
	Human IL-8 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.