ELISA binding curve showing dose-dependent interaction between anti-IL-8 antibody and recombinant human IL-8 protein with His tag
Human IL-8/CXCL8 Protein (IL8-HM1RA)
Human IL-8/CXCL8 Protein (IL8-HM1RA)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-8, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-8 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ser28-Ser99
Catalog #IL8-HM1RA
Regular price $346.50
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Human IL-8/CXCL8 Protein (IL8-HM1RA) Summary

Product Description

Recombinant Human IL-8/CXCL8 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser28-Ser99.

Product Background

Interleukin-8 (IL-8) has been revealed as a critical regulator of CNS function and development with participation in many CNS disorders including gliomas.Several promising approaches that target directly or indirectly IL-8 effects in gliomas are currently in progress while more-in-depth studies are needed to validate its biomarker role and elucidate the underlying molecular mechanisms.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human IL-8/CXCL8 Protein (IL8-HM1RA) Specifications/Details

Protein
IL-8/CXCL8
Synonyms
Interleukin-8; IL-8; GCP-1; MDNCF; MONAP; NAP-1; GCP/IL-8 protein IV; IL8/NAP1 form I; CXCL8; LECT; LUCT; LYNAP; NAF
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ser28-Ser99
Molecular Weight
The protein has a predicted MW of 9.5 kDa. Due to glycosylation, the protein migrates to 11-13 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human IL-8, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-8 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human IL-8, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-8 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. Immobilized Human IL-8, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-8 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.
Human IL-8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-8 is greater than 95% as determined by SEC-HPLC. The purity of Human IL-8 is greater than 95% as determined by SEC-HPLC.
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