Human IL-8/CXCL8 Protein (IL8-HM1RA)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-8, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-8 Antibody, hFc Tag with the EC50 of 12.5ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ser28-Ser99

Catalog: IL8-HM1RA

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Product Description Recombinant Human IL-8/CXCL8 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser28-Ser99.
Product Category Recombinant Protein
Target IL-8/CXCL8
Synonyms Interleukin-8; IL-8; GCP-1; MDNCF; MONAP; NAP-1; GCP/IL-8 protein IV; IL8/NAP1 form I; CXCL8; LECT; LUCT; LYNAP; NAF
Gene/Protein ID P10145-1
Species Human
Exact Sequence Ser28-Ser99
Molecular Weight The protein has a predicted MW of 9.5 kDa. Due to glycosylation, the protein migrates to 11-13 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human IL-8, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-8 Antibody, hFc Tag with the EC50 of 12.5ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-8, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-8 Antibody, hFc Tag with the EC50 of 12.5ng/ml determined by ELISA.
The purity of Human IL-8 is greater than 95% as determined by SEC-HPLC.
Human IL-8 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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