Product Description |
Recombinant Human IL-9 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln19-Ile144. |
Product Background |
IL-9 is a pleiotropic cytokine that influences various distinct functions of different target cells such as T cells, B cells, mast cells and airway epithelial cells by activating STAT1, STAT3 and STAT5. Because of its pleiotropic functions, IL-9 has been demonstrated to be involved in several diseases, such as cancer, autoimmunity and other pathogen-mediated immune-regulated diseases. In this review, we focus on the role of Th9 and IL-9-producing cells in allergic asthma. |
Product Category |
Recombinant Protein |
Target |
IL-9 |
Synonyms |
Interleukin-9; IL-9; Cytokine P40; T-cell growth factor P40; HP40; P40 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln19-Ile144 |
Molecular Weight |
The protein has a predicted MW of 15.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-9, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-IL-9 Anitibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human IL-9, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-IL-9 Anitibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). |
|
|
Human IL-9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50-for this effect is < 0.3 ng/mL (QC Test). |
|
|
The purity of Human IL-9 is greater than 95% as determined by SEC-HPLC. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recently viewed
