Human IL-9 Protein (IL9-HM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-9, His Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-IL-9 Anitibody, hFc Tag with the EC50 of 13.6ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gln19-Ile144

Catalog: IL9-HM101

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Product Description	Recombinant Human IL-9 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln19-Ile144.
Product Background	IL-9 is a pleiotropic cytokine that influences various distinct functions of different target cells such as T cells, B cells, mast cells and airway epithelial cells by activating STAT1, STAT3 and STAT5. Because of its pleiotropic functions, IL-9 has been demonstrated to be involved in several diseases, such as cancer, autoimmunity and other pathogen-mediated immune-regulated diseases. In this review, we focus on the role of Th9 and IL-9-producing cells in allergic asthma.
Product Category	Recombinant Protein

Target	IL-9
Gene/Protein ID	P15248
Species	Human
Exact Sequence	Gln19-Ile144
Molecular Weight	The protein has a predicted MW of 15.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human IL-9, His Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-IL-9 Anitibody, hFc Tag with the EC50 of 13.6ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human IL-9, His Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-IL-9 Anitibody, hFc Tag with the EC50 of 13.6ng/ml determined by ELISA.
	Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50-for this effect is < 0.3 ng/mL.
	The purity of Human IL-9 is greater than 95% as determined by SEC-HPLC.
	Human IL-9 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.