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Human IL-9 Protein, Ultra Low Endotoxin (IL9-HM101-UL) Summary
Product Description |
Recombinant Human IL-9 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln19-Ile144. |
Product Background |
IL-9 is a pleiotropic cytokine that influences various distinct functions of different target cells such as T cells, B cells, mast cells and airway epithelial cells by activating STAT1, STAT3 and STAT5. Because of its pleiotropic functions, IL-9 has been demonstrated to be involved in several diseases, such as cancer, autoimmunity and other pathogen-mediated immune-regulated diseases. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human IL-9 Protein, Ultra Low Endotoxin (IL9-HM101-UL) Specifications/Details
Protein |
IL-9 |
Synonyms |
Interleukin-9; IL-9; Cytokine P40; T-cell growth factor P40; HP40; P40 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln19-Ile144 |
Molecular Weight |
The protein has a predicted MW of 15.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-9, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-IL-9 Anitibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human IL-9, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-IL-9 Anitibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). |
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Human IL-9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50-for this effect is < 1 ng/mL (QC Test). |
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The purity of Human IL-9 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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