Regular price
$420.00
Order by 4pm ET for delivery on:
Non-biotinylated Human Integrin alpha 4 beta 1 (ITGA4&ITGB1) Heterodimer Protein (ITG-HM441) Summary
Product Description |
Recombinant Human Integrin alpha 4 beta 1 (ITGA4&ITGB1) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr34-Thr977(ITGA4) acidic tail and Gln21-Asp728(ITGB1) basic tail. |
Product Background |
Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Non-biotinylated Human Integrin alpha 4 beta 1 (ITGA4&ITGB1) Heterodimer Protein (ITG-HM441) Specifications/Details
Protein |
Integrin alpha 4 beta 1 (ITGA4&ITGB1) Heterodimer |
Synonyms |
Integrin alpha 4 beta 1; beta1; alpha 4; ITGA4&ITGB1;Integrin alpha 4 β 1; β1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Tyr34-Thr977(ITGA4) acidic tail & Gln21-Asp728(ITGB1) basic tail |
Molecular Weight |
The protein has a predicted MW of 112.5 kDa (ITGA4) and 83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 70-90 kDa and 120-160 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human ITGA4&ITGB1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGA4 Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Human ITGA4&ITGB1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGA4 Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA. |
|
|
Human ITGA4&ITGB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human ITGA4&ITGB1 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
