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Human Integrin alpha 4 beta 7 (ITGA4&ITGB7) Heterodimer Protein (ITG-HM147) Summary
Product Description |
Recombinant Human Integrin alpha 4 beta 7 (ITGA4&ITGB7) Heterodimer Protein is expressed as a heterodimer of ITGA4 and ITGB7 by co-expression in HEK293. Subunit ITGA4 is fused with a His tag at the C-terminus and subunit ITGB7 contains no tag.It contains Tyr34-Thr977 (ITGA4) acidic tail and Glu20-His723 (ITGB7) basic tail. |
Product Background |
Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human Integrin alpha 4 beta 7 (ITGA4&ITGB7) Heterodimer Protein (ITG-HM147) Specifications/Details
Protein |
Integrin alpha 4 beta 7 (ITGA4&ITGB7) Heterodimer |
Synonyms |
Integrin alpha 4 beta 7; beta7; alpha 4; LPAM; ITGA4&ITGB7;Integrin alpha 4 β 7; β7 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Tyr34-Thr977(ITGA4) acidic tail & Glu20-His723(ITGB7) basic tail |
Molecular Weight |
The protein has a predicted MW of 111.18 kDa (ITGA4) and 81.3 kDa (ITGB7). Due to glycosylation, the protein migrates to 70-80 kDa, 80-95 kDa, 100-120 kDa and 140-160 kDa kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human ITGA4&ITGB7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGA4 Antibody, hFc Tag with the EC50 of 5.7/ng/ml determined by ELISA. The affinity constant of 45.41 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 85% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS, 8% trehalose (pH 7.4). |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human MADCAM1, hFc Tag (Cat. MCM-HM201) captured on CM5 Chip via Protein A can bind Human ITGA4&ITGB7, His Tag with an affinity constant of 45.41 nM as determined in SPR assay (Biacore S200). |
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Immobilized Human ITGA4&ITGB7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGA4 Antibody, hFc Tag with the EC50 of 5.7/ng/ml determined by ELISA. |
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Immobilized Human ITGA4&ITGB7, His Tag at 5ug/ml(100ul/well) on the plate. Dose response curve for Human MADCAM1, hFc Tag (Cat. MCM-HM201) with the EC50 of 9.2ng/ml determined by ELISA. |
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Human ITGA4&ITGB7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human ITGA4&ITGB7 is greater than 85% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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