Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein (ITG-HM451)
Catalog: ITG-HM451
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Product Description | Recombinant Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Phe42-Tyr995 (ITGA5) acidic tail&Gln21-Asp728 (ITGB1) basic tail . |
Product Category | Recombinant Protein |
Target | Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer |
Synonyms | Integrin alpha 5 beta 1; VLA-5; ITGA5&ITGB1 |
Gene/Protein ID | P08648 (ITGA5)&P05556-1 (ITGB1) |
Species | Human |
Exact Sequence | Phe42-Tyr995 (ITGA5) acidic tail&Gln21-Asp728 (ITGB1) basic tail |
Molecular Weight | The protein has a predicted MW of 111.8 kDa (ITGA5)&83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 100-140 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human ITGA5&ITGB1, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-ITGA5&ITGB1 Antibody, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA. See testing image for detail. |
Product Tag | C-His-Avi |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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