Name: Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein, Ultra Low Endotoxin (ITG-HM451-UL)
Brand: KactusBio
SKU: ITG-HM451-UL-100UG
Price: 454.0 USD
Availability: InStock

Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein, Ultra Low Endotoxin (ITG-HM451-UL)

Immobilized Human ITGA5&ITGB1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGA5&ITGB1 Antibody, hFc Tag with the EC50 of 16.6ng/ml determined by ELISA (QC Test).

Human ITGA5&ITGB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human ITGA5&ITGB1 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human ITGA5&ITGB1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGA5&ITGB1 Antibody, hFc Tag with the EC50 of 16.6ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Phe42-Tyr995(ITGA5) acidic tail & Gln21-Asp728(ITGB1) basic tail

Catalog #ITG-HM451-UL

Size

100UG

500UG

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Product Description	Recombinant Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus of ITGA5.It contains Phe42-Tyr995(ITGA5) acidic tail and Gln21-Asp728(ITGB1) basic tail.
Product Background	Integrin alpha 5/ beta 1, also known as VLA-5, is a widely expressed non-covalent heterodimer of a 160 kDa alpha 5 and a 130 kDa beta 1 Integrin subunit.Alpha 5/ beta 1 is up‑regulated on tumor vasculature and promotes angiogenesis.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer
Synonyms	Integrin alpha 5 beta 1; VLA-5; ITGA5&ITGB1;Integrin alpha 5 β 1; α5β1
Accession	P08648(ITGA5)&P05556-1(ITGB1)
Species	Human
Biotinylated	no
Amino Acid Range	Phe42-Tyr995(ITGA5) acidic tail & Gln21-Asp728(ITGB1) basic tail
Molecular Weight	The protein has a predicted MW of 111.8 kDa (ITGA5) & 83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 100-140 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human ITGA5&ITGB1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGA5&ITGB1 Antibody, hFc Tag with the EC50 of 16.6ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human ITGA5&ITGB1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGA5&ITGB1 Antibody, hFc Tag with the EC50 of 16.6ng/ml determined by ELISA (QC Test).
	Human ITGA5&ITGB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human ITGA5&ITGB1 is greater than 95% as determined by SEC-HPLC.