Human Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer Protein (ITG-HM1MB)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human ITGAM&ITGB2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC50 of 13.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Phe17-Asn1104(ITGAM)acidic tail & Gln23-Asn700(ITGB2)basic tail

Catalog: ITG-HM1MB

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Product Description Recombinant Human Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe17-Asn1104(ITGAM) acidic tail&Gln23-Asn700(ITGB2) basic tail.

Product Background

Integrin αMβ2, also called Mac-1 or complement receptor-3 (CR3), is a heterodimer expressed on monocytes. It functions as both an adhesion molecule mediating the diapedesis of leukocytes across the endothelium and a receptor for the iC3b fragment of complement responsible for phagocytic/degranulation responses to microorganisms[1]. Active αMβ2 binds iC3b, coagulation proteins fibrinogen, plasminogen and factor X, extracellular matrix (ECM) proteins fibronectin, laminin and collagen, and cell surface ICAMs, myelin basic protein and DC­SIGN[2].

Product Category

Recombinant Protein

Target

Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer

Gene/Protein ID

P11215-1(ITGAM)&P05107-1(ITGB2)

Species

Human

Exact Sequence

Phe17-Asn1104(ITGAM)acidic tail & Gln23-Asn700(ITGB2)basic tail
Molecular Weight The protein has a predicted MW of 126.10 kDa(ITGAM)&79.50 kDa(ITGB2). Due to glycosylation, the protein migrates to 140-160 kDa (ITGAM)&90-100 kDa (ITGB2) based on Tris-Bis PAGE result.

Activity

Immobilized Human ITGAM&ITGB2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC50 of 13.7ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human ITGAM&ITGB2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC50 of 13.7ng/ml determined by ELISA.
The purity of Human ITGAM&ITGB2 is greater than 95% as determined by SEC-HPLC.
Human ITGAM&ITGB2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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