Name: Human Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer Protein (ITG-HM1MB)
Brand: KactusBio
SKU: ITG-HM1MB-100UG
Price: 412.5 USD
Availability: InStock

Human Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer Protein (ITG-HM1MB)

Immobilized Human ITGAM&ITGB2, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC50 of 11.9 ng/ml determined by ELISA (QC Test).

Human ITGAM&ITGB2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human ITGAM&ITGB2, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC50 of 11.9 ng/ml determined by ELISA (QC Test).
Amino Acid Range: Phe17-Asn1104 (ITGAM) acidic tail & Gln23-Asn700 (ITGB2) basic tail

Catalog #ITG-HM1MB

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Human Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer Protein (ITG-HM1MB) Summary

Product Description	Recombinant Human Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus of ITGAM; It contains Phe17-Asn1104 (ITGAM) acidic tail and Gln23-Asn700 (ITGB2) basic tail.
Product Background	Integrin αMβ2, also called Mac-1 or complement receptor-3 (CR3), is a heterodimer expressed on monocytes. It functions as both an adhesion molecule mediating the diapedesis of leukocytes across the endothelium and a receptor for the iC3b fragment of complement responsible for phagocytic/degranulation responses to microorganisms[1]. Active αMβ2 binds iC3b, coagulation proteins fibrinogen, plasminogen and factor X, extracellular matrix (ECM) proteins fibronectin, laminin and collagen, and cell surface ICAMs, myelin basic protein and DCSIGN[2].
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer Protein (ITG-HM1MB) Specifications/Details

Protein	Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer
Synonyms	αMβ2; ITGAM&ITGB2; ITGAM; ITGB2
Accession	P11215-1(ITGAM)&P05107-1(ITGB2)
Species	Human
Biotinylated	no
Amino Acid Range	Phe17-Asn1104 (ITGAM) acidic tail & Gln23-Asn700 (ITGB2) basic tail
Molecular Weight	The protein has a predicted MW of 126.10 kDa (ITGAM) and 79.50 kDa (ITGB2). Due to glycosylation, the protein migrates to 140-160 kDa (ITGAM) and 90-100 kDa (ITGB2) based on Bis-Tris PAGE result.
Activity	Immobilized Human ITGAM&ITGB2, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC50 of 11.9 ng/ml determined by ELISA (QC Test).
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human ITGAM&ITGB2, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC50 of 11.9 ng/ml determined by ELISA (QC Test).
	Human ITGAM&ITGB2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.