Human Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer Protein (ITG-HM1MB)
Human Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer Protein (ITG-HM1MB)
Human Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer Protein (ITG-HM1MB)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human ITGAM&ITGB2, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC50 of 11.9 ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Phe17-Asn1104 (ITGAM) acidic tail & Gln23-Asn700 (ITGB2) basic tail
Catalog #ITG-HM1MB
Regular price $375.00
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Product Description

Recombinant Human Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus; It contains Phe17-Asn1104 (ITGAM) acidic tail and Gln23-Asn700 (ITGB2) basic tail.

Product Background

Integrin αMβ2, also called Mac-1 or complement receptor-3 (CR3), is a heterodimer expressed on monocytes. It functions as both an adhesion molecule mediating the diapedesis of leukocytes across the endothelium and a receptor for the iC3b fragment of complement responsible for phagocytic/degranulation responses to microorganisms[1]. Active αMβ2 binds iC3b, coagulation proteins fibrinogen, plasminogen and factor X, extracellular matrix (ECM) proteins fibronectin, laminin and collagen, and cell surface ICAMs, myelin basic protein and DC­SIGN[2].

Product Category

Recombinant Protein / Antibody discovery

Protein

Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer

Synonyms

αMβ2; ITGAM&ITGB2; ITGAM; ITGB2

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Phe17-Asn1104 (ITGAM) acidic tail & Gln23-Asn700 (ITGB2) basic tail

Molecular Weight

The protein has a predicted MW of 126.10 kDa (ITGAM) and 79.50 kDa (ITGB2). Due to glycosylation, the protein migrates to 140-160 kDa (ITGAM) and 90-100 kDa (ITGB2) based on Bis-Tris PAGE result.

Activity

Immobilized Human ITGAM&ITGB2, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC50 of 11.9 ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human ITGAM&ITGB2, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC50 of 11.9 ng/ml determined by ELISA (QC Test). Immobilized Human ITGAM&ITGB2, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC50 of 11.9 ng/ml determined by ELISA (QC Test).
Human ITGAM&ITGB2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human ITGAM&ITGB2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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