- Express System: HEK293
- Product Tag: C-His-Avi
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Human ITGAV&ITGB1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-alpha V Antibody, hFc Tag with the EC50 of 14.4ng/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Phe31-Val992 (ITGAV) acidic tail & Gln21-Asp728 (ITGB1) basic tail
Product Description |
Recombinant Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe31-Val992 (ITGAV) acidic tail & Gln21-Asp728 (ITGB1) basic tail. |
Product Background |
Integrins are transmembrane heterodimeric receptors that contribute to diverse biological functions and play a conserved role in mediating cell adhesion to fixed extracellular ligands and in the maintenance of tissue integrity. αVβ1 is the major integrin on several different primary fibroblasts responsible for binding to TGFb1 LAP and for mediating activation of latent TGFb by these cells. |
Product Category |
Recombinant Protein |
Target |
Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Phe31-Val992 (ITGAV) acidic tail & Gln21-Asp728 (ITGB1) basic tail |
Molecular Weight |
The protein has a predicted MW of 114 kDa (ITGAV) and 83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 130-160 kDa (ITGAV) and 100-120 kDa (ITGB1) based on Bis-Tris PAGE result. |
Activity |
Immobilized Human ITGAV&ITGB1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-alpha V Antibody, hFc Tag with the EC50 of 14.4ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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