Name: Non-biotinylated Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein (ITG-HM4V1)
Brand: KactusBio
SKU: ITG-HM4V1-100UG
Price: 412.5 USD
Availability: InStock

Non-biotinylated Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein (ITG-HM4V1)

Immobilized Human ITGAV&ITGB1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGAV Antibody, hFc Tag with the EC50 of 18.9ng/ml determined by ELISA (QC Test).

Human ITGAV&ITGB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Anti-ITGAV Ab., hFc Tag on ProA-Biosensor can bind Human ITGAV&ITGB1, His-Avi Tag with an affinity constant of 0.13 µM as determined in BLI assay (Gator® Prime).

The purity of Human ITGAV&ITGB1 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human ITGAV&ITGB1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGAV Antibody, hFc Tag with the EC50 of 18.9ng/ml determined by ELISA (QC Test).
Amino Acid Range: Phe31-Val992 (ITGAV) acidic tail & Gln21-Asp728 (ITGB1) basic tail

Catalog #ITG-HM4V1

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Non-biotinylated Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein (ITG-HM4V1) Summary

Product Description	Recombinant Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus of ITGAV.It contains Phe31-Val992 (ITGAV) acidic tail and Gln21-Asp728 (ITGB1) basic tail.
Product Background	Integrins are transmembrane heterodimeric receptors that contribute to diverse biological functions and play a conserved role in mediating cell adhesion to fixed extracellular ligands and in the maintenance of tissue integrity. αVβ1 is the major integrin on several different primary fibroblasts responsible for binding to TGFb1 LAP and for mediating activation of latent TGFb by these cells.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Non-biotinylated Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein (ITG-HM4V1) Specifications/Details

Protein	Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer
Synonyms	ITGAV&ITGB1; αVβ1; Integrin alpha V beta 1; Integrin alpha V β 1
Accession	P06756-1(ITGAV)&P05556-1(ITGB1)
Species	Human
Biotinylated	no
Amino Acid Range	Phe31-Val992 (ITGAV) acidic tail & Gln21-Asp728 (ITGB1) basic tail
Molecular Weight	The protein has a predicted MW of 114 kDa (ITGAV) and 83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 130-160 kDa (ITGAV) and 100-120 kDa (ITGB1) based on Bis-Tris PAGE result.
Activity	Immobilized Human ITGAV&ITGB1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGAV Antibody, hFc Tag with the EC50 of 18.9ng/ml determined by ELISA (QC Test).
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human ITGAV&ITGB1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGAV Antibody, hFc Tag with the EC50 of 18.9ng/ml determined by ELISA (QC Test).
	Human ITGAV&ITGB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Anti-ITGAV Ab., hFc Tag on ProA-Biosensor can bind Human ITGAV&ITGB1, His-Avi Tag with an affinity constant of 0.13 µM as determined in BLI assay (Gator® Prime).
	The purity of Human ITGAV&ITGB1 is greater than 95% as determined by SEC-HPLC.