• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human ITGAV&ITGB6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Avi Tag (Cat. TG1-HM401B) with the EC50 of 55.3ng/ml determined by ELISA.
  • Amino Acid Range: Phe31-Val992(ITGAV) acidic tail & Gly22-Asn707(ITGB6) basic tail
Catalog #ITG-HM1A6
Price
Regular price $413.00
Regular price Sale price $413.00
/
Size
Avantor VWR Fisher Scientific

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Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein (ITG-HM1A6) Summary

Product Description

 Recombinant Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein is expressed as a heterodimer of ITGAV and ITGB6 by co-expression in HEK293. Subunit ITGAV is fused with an acidic tail and a His tag at the C-terminus and subunit ITGB6 is fused with an basic tail at the C-terminus.It contains Phe31-Val992 (ITGAV) acidic tail and Gly22-Asn707 (ITGB6) basic tail.

Product Background

 ITGAV&ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. ITGAV&ITGB6 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1.

Product Category

 Recombinant Protein / Antibody discovery / ADC targets

Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein (ITG-HM1A6) Specifications/Details

Protein
 Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer
Synonyms
 αVβ6; Integrin alpha V beta 6; ITGAV&ITGB6;Integrin alpha V β 6
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Phe31-Val992(ITGAV) acidic tail & Gly22-Asn707(ITGB6) basic tail
Molecular Weight
 The protein has a predicted MW of 112.21 kDa (ITGAV) and 79.1 kDa (ITGB6). Due to glycosylation, the protein migrates to 95-130 kDa and 140-160 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human ITGAV&ITGB6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Avi Tag (Cat. TG1-HM401B) with the EC50 of 55.3ng/ml determined by ELISA.
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human ITGAV&ITGB6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Avi Tag (Cat. TG1-HM401B) with the EC50 of 55.3ng/ml determined by ELISA. Immobilized Human ITGAV&ITGB6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Avi Tag (Cat. TG1-HM401B) with the EC50 of 55.3ng/ml determined by ELISA.
Immobilized Human ITGAV&ITGB6, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGAV&ITGB6 Antibody, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA. Immobilized Human ITGAV&ITGB6, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGAV&ITGB6 Antibody, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA.
Immobilized Human ITGAV&ITGB6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGAV Antibody, hFc Tag with the EC50 of 6.8ng/ml determined by ELISA. Immobilized Human ITGAV&ITGB6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGAV Antibody, hFc Tag with the EC50 of 6.8ng/ml determined by ELISA.
Immobilized Human ITGAV&ITGB6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGB6 Antibody, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA. Immobilized Human ITGAV&ITGB6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGB6 Antibody, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA.
HumanITGAV&ITGB6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. HumanITGAV&ITGB6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Anti-ITGAV&ITGB6 Antibody on ProA-Biosensor can bind Human ITGAV&ITGB6, His Tag with an affinity constant of 17.20 nM as determined in BLI assay. Loaded Anti-ITGAV&ITGB6 Antibody on ProA-Biosensor can bind Human ITGAV&ITGB6, His Tag with an affinity constant of 17.20 nM as determined in BLI assay.
The purity of HumanITGAV&ITGB6 is greater than 95% as determined by SEC-HPLC. The purity of HumanITGAV&ITGB6 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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