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Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein (ITG-HM1A6) Summary
Product Description |
Recombinant Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein is expressed as a heterodimer of ITGAV and ITGB6 by co-expression in HEK293. Subunit ITGAV is fused with an acidic tail and a His tag at the C-terminus and subunit ITGB6 is fused with an basic tail at the C-terminus.It contains Phe31-Val992 (ITGAV) acidic tail and Gly22-Asn707 (ITGB6) basic tail. |
Product Background |
ITGAV&ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. ITGAV&ITGB6 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein (ITG-HM1A6) Specifications/Details
Protein |
Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer |
Synonyms |
αVβ6; Integrin alpha V beta 6; ITGAV&ITGB6;Integrin alpha V β 6 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Phe31-Val992(ITGAV) acidic tail & Gly22-Asn707(ITGB6) basic tail |
Molecular Weight |
The protein has a predicted MW of 112.21 kDa (ITGAV) and 79.1 kDa (ITGB6). Due to glycosylation, the protein migrates to 95-130 kDa and 140-160 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human ITGAV&ITGB6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Avi Tag (Cat. TG1-HM401B) with the EC50 of 55.3ng/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human ITGAV&ITGB6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Avi Tag (Cat. TG1-HM401B) with the EC50 of 55.3ng/ml determined by ELISA. |
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Immobilized Human ITGAV&ITGB6, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGAV&ITGB6 Antibody, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA. |
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HumanITGAV&ITGB6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Anti-ITGAV&ITGB6 Antibody on ProA-Biosensor can bind Human ITGAV&ITGB6, His Tag with an affinity constant of 17.20 nM as determined in BLI assay. |
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The purity of HumanITGAV&ITGB6 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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