Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein (ITG-HM4V8)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human ITGAV&ITGB8, His Tag at 5_g/ml (100_l/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 0.39ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Phe31-Val992(ITGAV) & Glu43-Arg684(ITGB8)

Catalog: ITG-HM4V8

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Product Description Recombinant Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Phe31-Val992(ITGAV) acidic tail&Glu43-Arg684(ITGB8) basic tail.
Product Category Recombinant Protein
Target Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer
Synonyms Integrin alpha V beta 8; TGAV&ITGB8
Gene/Protein ID P06756-1(ITGAV)&P26012-1(ITGB8)
Species Human
Exact Sequence Phe31-Val992(ITGAV) & Glu43-Arg684(ITGB8)
Molecular Weight The protein has a predicted MW of 114 kDa (ITGAV)&76 kDa (ITGB8). Due to glycosylation, the protein migrates to 90-150 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human ITGAV&ITGB8, His Tag at 5_g/ml (100_l/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 0.39ug/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per _g by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human ITGAV&ITGB8, His Tag at 5_g/ml (100_l/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 0.39ug/ml determined by ELISA.
The purity of Human ITGAV&ITGB8 is greater than 95% as determined by SEC-HPLC.
Human ITGAV&ITGB8 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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