HPLC chromatogram with sharp protein peak at 10.055 minutes indicating high purity and minimal aggregation.
Human Interferon omega-1 Protein (IFN-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 20.96 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Leu22-Ser195
Catalog #IFN-HM101
Regular price $297.00
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Human Interferon omega-1 Protein (IFN-HM101) Summary

Product Description

Recombinant Human Interferon omega-1 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Leu22-Ser195.

Product Background

Human Interferon omega-1, a type I IFN, also known as IFN-ω1 or IFNW1, it contains a potential N-glycosylation site like human IFN-β. IFN-ω1 binds to the interferon alpha/beta receptor but not to the interferon gamma receptor. All IFN-ω genes analysed to date are devoid of introns. IFN-ω1 has been shown to be well-tolerated in man and to induce reductions of hepatitis C virus RNA levels in a series of human clinical trials.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human Interferon omega-1 Protein (IFN-HM101) Specifications/Details

Protein
Interferon omega-1
Synonyms
Interferon alpha-II-1; IFNW1; IFN-ω1; IFN-omega-1
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Leu22-Ser195
Molecular Weight
The protein has a predicted MW of 21.25 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 20.96 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human IFNAR1, His Tag immobilized on CM5 Chip can bind Human Interferon omega-1, His Tag with an affinity constant of 20.96 uM as determined in SPR assay (Biacore T200). Human IFNAR1, His Tag immobilized on CM5 Chip can bind Human Interferon omega-1, His Tag with an affinity constant of 20.96 uM as determined in SPR assay (Biacore T200).
Human Interferon omega-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Interferon omega-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Interferon omega-1 is greater than 95% as determined by SEC-HPLC. The purity of Human Interferon omega-1 is greater than 95% as determined by SEC-HPLC.
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