Product Description |
Recombinant Human ITCH Protein is expressed from E.coli without tag.It contains Arg485-Glu862. |
Product Background |
Itch or itchy E3 is a member of the homologous to E6-associated protein C-terminus (HECT)-type family of E3 ligases, with the protein-interacting WW-domains for the recruitment of substrate and the HECT domain for the transfer of ubiquitin to the substrate, and its deletion results in an itchy phenotype with constant skin scratching and multi-organ inflammation. |
Product Category |
Recombinant Protein |
Target |
ITCH |
Synonyms |
AIP4; NAPP1; AIF4 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Arg485-Glu862 |
Molecular Weight |
The protein has a predicted MW of 44.98 kDa same as Bis-Tris PAGE result. |
Product Tag |
No Tag |
Express System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS, 200mM L-Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human ITCH on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human ITCH is greater than 90% as determined by SEC-HPLC. |
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