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$330.00
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Human ITCH Protein (ITH-HE001) Summary
Product Description |
Recombinant Human ITCH Protein is expressed from E.coli without tag.It contains Arg485-Glu862. |
Product Background |
Itch or itchy E3 is a member of the homologous to E6-associated protein C-terminus (HECT)-type family of E3 ligases, with the protein-interacting WW-domains for the recruitment of substrate and the HECT domain for the transfer of ubiquitin to the substrate, and its deletion results in an itchy phenotype with constant skin scratching and multi-organ inflammation. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human ITCH Protein (ITH-HE001) Specifications/Details
Protein |
ITCH |
Synonyms |
AIP4; NAPP1; AIF4 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Arg485-Glu862 |
Molecular Weight |
The protein has a predicted MW of 44.98 kDa same as Bis-Tris PAGE result. |
Product Tag |
No Tag |
Fluorophore |
Unconjugated |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS, 200mM L-Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human ITCH on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human ITCH is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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