Product Description |
Recombinant Human JAM-A Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser28-Val238. |
Product Background |
junctional adhesion molecule A (JAM-A), a cell adhesion molecule, is highly elevated in human GBM cancer stem cells and predicts poor patient prognosis. While JAM-A is also highly expressed in other cells in the tumor microenvironment, specifically microglia and macrophages,JAM-A functions to suppress pathogenic microglial activation in the female tumor microenvironment, highlighting an emerging role for sex differences in the GBM microenvironment and suggesting that sex differences extend beyond previously reported tumor cell-intrinsic differences. |
Product Category |
Recombinant Protein |
Target |
JAM-A |
Synonyms |
JAM-A; JAM-1; CD321; JAM; JCAM; JCAM1; KAT; PAM-1; F11 receptor; F11R; PAM-1KAT |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser28-Val238. |
Molecular Weight |
The protein has a predicted MW of 24 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human JAM-A, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-JAM-A Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human JAM-A, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-JAM-A Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. |
|
|
Human JAM-A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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