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Human JAM-A Protein, Ultra Low Endotoxin (JAM-HM10A-UL) Summary
Product Description |
Recombinant Human JAM-A Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser28-Val238. |
Product Background |
junctional adhesion molecule A (JAM-A), a cell adhesion molecule, is highly elevated in human GBM cancer stem cells and predicts poor patient prognosis. While JAM-A is also highly expressed in other cells in the tumor microenvironment, specifically microglia and macrophages,JAM-A functions to suppress pathogenic microglial activation in the female tumor microenvironment, highlighting an emerging role for sex differences in the GBM microenvironment and suggesting that sex differences extend beyond previously reported tumor cell-intrinsic differences. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human JAM-A Protein, Ultra Low Endotoxin (JAM-HM10A-UL) Specifications/Details
Protein |
JAM-A |
Synonyms |
JAM-A; JAM-1; CD321; JAM; JCAM; JCAM1; KAT; PAM-1; F11 receptor; F11R; PAM-1KAT |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser28-Val238. |
Molecular Weight |
The protein has a predicted MW of 24 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human JAM-A, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-JAM-A Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Human JAM-A, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-JAM-A Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. |
|
|
Human JAM-A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
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