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Human JAML Protein (JAM-HM101) Summary
Product Description |
Recombinant Human JAML Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu20-Leu275. |
Product Background |
JAML (junctional adhesion molecule-like), contains 2 extracellular immunoglobulin-like domains, a transmembrane segment, and a cytoplasmic tail. JAML mRNA is expressed in hematopoietic tissues and is prominently expressed in granulocytes. The fact that JAML protein is localized at the cell plasma membrane in the areas of cell-cell contacts, whereas it is not detected at free cell borders, suggests that JAML is engaged in homophilic interactions. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human JAML Protein (JAM-HM101) Specifications/Details
Protein |
JAML |
Synonyms |
JunctioJAML; AMICA1; AMICA; CREA7-1; Gm638; JAML |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu20-Leu275 |
Molecular Weight |
The protein has a predicted MW of 30.1 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human JAML on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
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