Name: Human Kallikrein 1/KLK1 Protein (active form) (KLK-HM101)
Brand: KactusBio
SKU: KLK-HM101-100UG
Price: 375.0 USD
Availability: InStock

Human Kallikrein 1/KLK1 Protein (active form) (KLK-HM101)

Immobilized Human Kallikrein 1 (active form), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Kallikrein 1 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA.

Human Kallikrein 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human Kallikrein 1 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Kallikrein 1 (active form), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Kallikrein 1 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate: Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC). The specific activity is >8000 pmol/min/µg.
Amino Acid Range: Ile25-Ser262
Other Available Proteins:

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Catalog #KLK-HM101

Size

100UG

500UG

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Human Kallikrein 1/KLK1 Protein (active form) (KLK-HM101) Summary

Product Description	Recombinant Human Kallikrein 1/KLK1 Protein (active form) is expressed from HEK293 with His tag at the C-terminus.It contains Ile25-Ser262.
Product Background	KLK1 (tissue kallikrein 1) is a member of the tissue kallikrein family of serine proteases, which, in humans, comprises 15 proteases and constitutes the largest contiguous cluster of any family of proteases on the human genome. KLK1 cleaves both low-molecular-mass kininogen and HMMK (high-molecular-mass kininogen) at two sites to release the decapeptide kinin Lys-bradykinin (Lys-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg).
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human Kallikrein 1/KLK1 Protein (active form) (KLK-HM101) Specifications/Details

Protein	Kallikrein 1/KLK1
Synonyms	Kallikrein-1; KLKR; KLK1
Accession	P06870-1
Species	Human
Biotinylated	no
Amino Acid Range	Ile25-Ser262
Molecular Weight	The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human Kallikrein 1 (active form), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Kallikrein 1 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate: Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC). The specific activity is >8000 pmol/min/µg.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 50mM Tris, 150mM NaCl, 8% trehalose (pH 7.5).
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human Kallikrein 1 (active form), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Kallikrein 1 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA.
	Human Kallikrein 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human Kallikrein 1 is greater than 95% as determined by SEC-HPLC.

Human Kallikrein 1/KLK1 Protein (active form) (KLK-HM101) Summary

Product Description

Product Background

Product Category

Human Kallikrein 1/KLK1 Protein (active form) (KLK-HM101) Specifications/Details

Fluorophore

Shipping, Reconstitution, & Storage

Validation Images

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