Human Kallikrein 2/KLK2 Protein (active form) (KLK-HM002)
Human Kallikrein 2/KLK2 Protein (active form) (KLK-HM002)
  • Express System: HEK293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human Kallikrein 2, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Kallikrein 2 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate: PFR-AMC. The specific activity is >450 pmol/min/µg.
  • Amino Acid Range: Ile25-Pro261
Catalog #KLK-HM002
Regular price $675.00
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Human Kallikrein 2/KLK2 Protein (active form) (KLK-HM002) Summary

Product Description

Recombinant Human Kallikrein 2/KLK2 Protein (active form) is expressed from HEK293 without tag.It contains Ile25-Pro261.

Product Background

Kallikrein-related peptidase 2 (KLK2) is a serine protease exhibiting antiangiogenic properties through proteolytic activity. KLK2 is overexpressed in prostate cancer and plays a pivotal role in cancer progression, establishing it as a potential therapeutic target.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human Kallikrein 2/KLK2 Protein (active form) (KLK-HM002) Specifications/Details

Protein
Kallikrein 2/KLK2
Synonyms
KLK2; Kallikrein 2; hGK-1; hK2; KLK2A2
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ile25-Pro261
Molecular Weight
The protein has a predicted MW of 26.15 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human Kallikrein 2, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Kallikrein 2 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate: PFR-AMC. The specific activity is >450 pmol/min/µg.
Product Tag
No Tag
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in 20mM Tris, 150mM NaCl, 8% trehalose, 0.05% Brij-35, 5mM Benzamidine (pH 8.0).
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human Kallikrein 2, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Kallikrein 2 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. Immobilized Human Kallikrein 2, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Kallikrein 2 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA.
Human Kallikrein 2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Kallikrein 2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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