- Express System: HEK293
- Product Tag: No Tag
- Purity: > 95% as determined by Bis-Tris PAGE
- Activity: Immobilized Human Kallikrein 2, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Kallikrein 2 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate: PFR-AMC. The specific activity is >450 pmol/min/µg.
- Amino Acid Range: Ile25-Pro261
Human Kallikrein 2/KLK2 Protein (active form) (KLK-HM002) Summary
Product Description |
Recombinant Human Kallikrein 2/KLK2 Protein (active form) is expressed from HEK293 without tag.It contains Ile25-Pro261. |
Product Background |
Kallikrein-related peptidase 2 (KLK2) is a serine protease exhibiting antiangiogenic properties through proteolytic activity. KLK2 is overexpressed in prostate cancer and plays a pivotal role in cancer progression, establishing it as a potential therapeutic target. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human Kallikrein 2/KLK2 Protein (active form) (KLK-HM002) Specifications/Details
Protein |
Kallikrein 2/KLK2 |
Synonyms |
KLK2; Kallikrein 2; hGK-1; hK2; KLK2A2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ile25-Pro261 |
Molecular Weight |
The protein has a predicted MW of 26.15 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Kallikrein 2, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Kallikrein 2 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate: PFR-AMC. The specific activity is >450 pmol/min/µg. |
Product Tag |
No Tag |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 20mM Tris, 150mM NaCl, 8% trehalose, 0.05% Brij-35, 5mM Benzamidine (pH 8.0). |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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