Human Kallikrein 3/PSA-Protein (pro form) (KLK-HM103) Summary
Product Description |
Recombinant Human Kallikrein 3/PSA-Protein is expressed from HEK293 with His tag at the C-Terminus. The protein needs to be activated by Thermolysin for an activated form.It contains Ala18-Pro261. |
Product Background |
Kallikrein-related peptidase 3 (KLK3), also known as prostate-specific antigen (PSA), is the most useful biomarker for prostate cancer (PCa). KLK3 is suggested to play a role in regulating cancer growth through anti-angiogenic activity in vivo and in vitro. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human Kallikrein 3/PSA-Protein (pro form) (KLK-HM103) Specifications/Details
Protein |
Kallikrein 3/PSA |
Synonyms |
PSA; Kallikrein 3; Kallikrein-3; P-30 antigen; Semenogelase; KLK3; APS |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala18-Pro261 |
Molecular Weight |
The protein has a predicted MW of 27.93 kDa. Due to glycosylation, the protein migrates to 32-38 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Kallikrein 3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Kallikrein 3 Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p-Nitroanilide (Suc-RPY-pNA). The specific activity is >150 pmol/min/ug. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human Kallikrein 3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Kallikrein 3 Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA. |
|
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Human Kallikrein 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human Kallikrein 3 is greater than 95% as determined by SEC-HPLC. |
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