Human Kallikrein 5/KLK5 Protein (active form), Ultra Low Endotoxin (KLK-HM605-UL) Summary
Product Description |
Recombinant Human Kallikrein 5/KLK5 Protein is expressed from HEK293 with His tag and Flag tag at the C-terminus.It contains Ile67-Ser293. |
Product Background |
The inhibition of kallikrein 5 (KLK5) has been identified as a potential strategy for treatment of the genetic skin disorder Netherton syndrome, in which loss-of-function mutations in the SPINK5 gene lead to down-regulation of the endogenous inhibitor LEKTI-1 and profound skin-barrier defects with severe allergic manifestations. To aid in the development of a medicine for this target, an X-ray crystallographic system was developed to facilitate fragment-guided chemistry and knowledge-based drug-discovery approaches. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human Kallikrein 5/KLK5 Protein (active form), Ultra Low Endotoxin (KLK-HM605-UL) Specifications/Details
Protein |
Kallikrein 5/KLK5 |
Synonyms |
Kallikrein c; Klnc; KLK5; KLKL2; KLK-L2; SCTE |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ile67-Ser293 |
Molecular Weight |
The protein has a predicted MW of 31.7 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Human Kallikrein 5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >350 pmol/min/µg. |
Product Tag |
C-His-Flag |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 50mM MES, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in 50mM MES (pH 5.5). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human Kallikrein 5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. |
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Human Kallikrein 5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Anti-Kallikrein 5 Ab., hFc Tag on ProA-Biosensor can bind Human Kallikrein 5, His-Flag Tag with an affinity constant of 0.52 nM as determined in BLI assay (Gator® Prime). |
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The purity of Human Kallikrein 5 is greater than 95% as determined by SEC-HPLC. |
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