Human Kallikrein 5/KLK5 Protein (KLK-HM105)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Kallikrein 5, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Val23-Ser293

Catalog: KLK-HM105

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Product Description Recombinant Human Kallikrein 5/KLK5 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val23-Ser293.

Product Background

The inhibition of kallikrein 5 (KLK5) has been identified as a potential strategy for treatment of the genetic skin disorder Netherton syndrome, in which loss-of-function mutations in the SPINK5 gene lead to down-regulation of the endogenous inhibitor LEKTI-1 and profound skin-barrier defects with severe allergic manifestations. To aid in the development of a medicine for this target, an X-ray crystallographic system was developed to facilitate fragment-guided chemistry and knowledge-based drug-discovery approaches.

Product Category

Recombinant Protein

Target

Kallikrein 5/KLK5
Synonyms Kallikrein c; Klnc; KLK5; KLKL2; KLK-L2; SCTE

Gene/Protein ID

Q9Y337

Species

Human

Exact Sequence

Val23-Ser293
Molecular Weight The protein has a predicted MW of 30.7 kDa. Due to glycosylation, the protein migrates to 42-48 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human Kallikrein 5, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 50mM MES, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM MES, 150mM NaCl (pH 5.5).
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Kallikrein 5, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA.
The purity of Human Kallikrein 5 is greater than 95% as determined by SEC-HPLC.
Human Kallikrein 5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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