Human Kallikrein 5/KLK5 Protein (KLK-HM105)
Human Kallikrein 5/KLK5 Protein (KLK-HM105)
Human Kallikrein 5/KLK5 Protein (KLK-HM105)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Kallikrein 5, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Val23-Ser293
Catalog #KLK-HM105
Regular price $300.00
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Product Description
Recombinant Human Kallikrein 5/KLK5 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val23-Ser293.
Product Background
The inhibition of kallikrein 5 (KLK5) has been identified as a potential strategy for treatment of the genetic skin disorder Netherton syndrome, in which loss-of-function mutations in the SPINK5 gene lead to down-regulation of the endogenous inhibitor LEKTI-1 and profound skin-barrier defects with severe allergic manifestations. To aid in the development of a medicine for this target, an X-ray crystallographic system was developed to facilitate fragment-guided chemistry and knowledge-based drug-discovery approaches.
Product Category
Recombinant Protein
Target
Kallikrein 5/KLK5
Synonyms
Kallikrein c; Klnc; KLK5; KLKL2; KLK-L2; SCTE
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Val23-Ser293
Molecular Weight
The protein has a predicted MW of 30.7 kDa. Due to glycosylation, the protein migrates to 42-48 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human Kallikrein 5, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 50mM MES, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM MES, 150mM NaCl (pH 5.5).
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Kallikrein 5, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA.
Human Kallikrein 5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Kallikrein 5 is greater than 95% as determined by SEC-HPLC.

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