Product Description |
Recombinant Human Kallikrein 5/KLK5 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val23-Ser293. |
Product Background |
The inhibition of kallikrein 5 (KLK5) has been identified as a potential strategy for treatment of the genetic skin disorder Netherton syndrome, in which loss-of-function mutations in the SPINK5 gene lead to down-regulation of the endogenous inhibitor LEKTI-1 and profound skin-barrier defects with severe allergic manifestations. To aid in the development of a medicine for this target, an X-ray crystallographic system was developed to facilitate fragment-guided chemistry and knowledge-based drug-discovery approaches. |
Product Category |
Recombinant Protein |
Target |
Kallikrein 5/KLK5 |
Synonyms |
Kallikrein c; Klnc; KLK5; KLKL2; KLK-L2; SCTE |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Val23-Ser293 |
Molecular Weight |
The protein has a predicted MW of 30.7 kDa. Due to glycosylation, the protein migrates to 42-48 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Kallikrein 5, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 50mM MES, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM MES, 150mM NaCl (pH 5.5). |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human Kallikrein 5, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA. |
|
|
Human Kallikrein 5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human Kallikrein 5 is greater than 95% as determined by SEC-HPLC. |
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