Name: Human Kallikrein 5/KLK5 Protein (active form) (KLK-HM605)
Brand: KactusBio
SKU: KLK-HM605-100UG
Price: 412.5 USD
Availability: InStock

Human Kallikrein 5/KLK5 Protein (active form) (KLK-HM605)

Immobilized Human Kallikrein 5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.

Human Kallikrein 5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human Kallikrein 5 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Flag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Kallikrein 5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >350 pmol/min/µg.
Amino Acid Range: Ile67-Ser293

Catalog #KLK-HM605

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Human Kallikrein 5/KLK5 Protein (active form) (KLK-HM605) Summary

Product Description	Recombinant Human Kallikrein 5/KLK5 Protein is expressed from HEK293 with His tag and Flag tag at the C-terminus.It contains Ile67-Ser293.
Product Background	The inhibition of kallikrein 5 (KLK5) has been identified as a potential strategy for treatment of the genetic skin disorder Netherton syndrome, in which loss-of-function mutations in the SPINK5 gene lead to down-regulation of the endogenous inhibitor LEKTI-1 and profound skin-barrier defects with severe allergic manifestations. To aid in the development of a medicine for this target, an X-ray crystallographic system was developed to facilitate fragment-guided chemistry and knowledge-based drug-discovery approaches.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human Kallikrein 5/KLK5 Protein (active form) (KLK-HM605) Specifications/Details

Protein	Kallikrein 5/KLK5
Synonyms	Kallikrein c; Klnc; KLK5; KLKL2; KLK-L2; SCTE
Accession	Q9Y337
Species	Human
Biotinylated	no
Amino Acid Range	Ile67-Ser293
Molecular Weight	The protein has a predicted MW of 31.7 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human Kallikrein 5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >350 pmol/min/µg.
Product Tag	C-His-Flag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 50mM MES, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in 50mM MES, 150mM NaCl (pH 5.5). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human Kallikrein 5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.
	Human Kallikrein 5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human Kallikrein 5 is greater than 95% as determined by SEC-HPLC.