Human Kallikrein 6/KLK6 Protein (active form) (KLK-HM106)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Measured by its ability to cleave the fluorogenic peptide substrate: BOC-Gln-Ala-Arg-AMC. The specific activity is >1500 pmol/min/µg.
  • Amino Acid Range: Leu22-Lys244
  • Other Available Proteins:
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Catalog #KLK-HM106
Regular price $375.00
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Human Kallikrein 6/KLK6 Protein (active form) (KLK-HM106) Summary

Product Description

Recombinant Human Kallikrein 6/KLK6 Protein (active form) is expressed from HEK293 with His tag at the C-terminus.It contains Leu22-Lys244.

Product Background

Kallikrein 6 (KLK6), also referred to as kallikrein-related peptidase 6, is a member of a family of 15 serine proteases aligned in tandem on human chromosome 19q13.3-4. Of all kallikreins, KLK6 is the most abundant in the adult central nervous system (CNS). Within the CNS, KLK6 is expressed at the highest levels in the brain stem and spinal cord where expression is primarily associated with neurons and oligodendrocytes with a relative paucity of expression by astrocytes.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human Kallikrein 6/KLK6 Protein (active form) (KLK-HM106) Specifications/Details

Protein
Kallikrein 6/KLK6
Synonyms
Neurosin; Protease M; SP59; Zyme; KLK6; PRSS18; PRSS9
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Leu22-Lys244
Molecular Weight
The protein has a predicted MW of 26.11 kDa. Due to glycosylation, the protein migrates to 27-35 kDa based on Bis-Tris PAGE result.
Activity
Measured by its ability to cleave the fluorogenic peptide substrate: BOC-Gln-Ala-Arg-AMC. The specific activity is >1500 pmol/min/µg.
Product Tag
C-His

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in 20mM NaAC, 150mM NaCl, 8% trehalose, 0.05% Brij-35, 10mM Benzamidine (pH 5.0).
Reconstitution
Dissolve the lyophilized protein in 20mM NaAC (pH 5.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human Kallikrein 6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Kallikrein 6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Kallikrein 6 is greater than 95% as determined by SEC-HPLC. The purity of Human Kallikrein 6 is greater than 95% as determined by SEC-HPLC.
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