Human Kallikrein 6/KLK6 Protein (pro form) (KLK-HM116)

SKU: KLK-HM116-100UG

Human Kallikrein 6/KLK6 Protein (pro form) (KLK-HM116)

SKU: KLK-HM116-100UG
Human Kallikrein 6/KLK6 Protein (pro form) (KLK-HM116)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Measured by its ability to cleave the fluorogenic peptide substrate: BOC-Gln-Ala-Arg-AMC. The specific activity is >2000 pmol/min/µg.
  • Amino Acid Range: Glu17-Lys244
Catalog #KLK-HM116
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Size
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Human Kallikrein 6/KLK6 Protein (pro form) (KLK-HM116) Summary

Product Description

 Recombinant Human Kallikrein 6/KLK6 Protein (pro form) is expressed from HEK293 with His tag at the C-terminus. The protein needs to be activated by Lysyl-endopeptidase for an activated form.It contains Glu17-Lys244.

Product Background

 Kallikrein 6 (KLK6), also referred to as kallikrein-related peptidase 6, is a member of a family of 15 serine proteases aligned in tandem on human chromosome 19q13.3-4. Of all kallikreins, KLK6 is the most abundant in the adult central nervous system (CNS). Within the CNS, KLK6 is expressed at the highest levels in the brain stem and spinal cord where expression is primarily associated with neurons and oligodendrocytes with a relative paucity of expression by astrocytes.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human Kallikrein 6/KLK6 Protein (pro form) (KLK-HM116) Specifications/Details

Protein
 Kallikrein 6/KLK6
Synonyms
 Neurosin; Protease M; SP59; Zyme; KLK6; PRSS18; PRSS9
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Glu17-Lys244
Molecular Weight
 The protein has a predicted MW of 26.74 kDa. Due to glycosylation, the protein migrates to 27-35 kDa based on Bis-Tris PAGE result.
Activity
 Measured by its ability to cleave the fluorogenic peptide substrate: BOC-Gln-Ala-Arg-AMC. The specific activity is >2000 pmol/min/µg.
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 20mM NaAC, 150mM NaCl, 8% trehalose, 0.05% Brij-35, 10mM Benzamidine (pH 5.0).
Reconstitution
 Dissolve the lyophilized protein in 20mM NaAC (pH 5.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human Kallikrein 6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Kallikrein 6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Kallikrein 6 is greater than 95% as determined by SEC-HPLC. The purity of Human Kallikrein 6 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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