• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human KIR3DL2, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-KIR3DL2 Antibody, hFc Tag with the EC50 of 60.5ng/ml determined by ELISA.
  • Amino Acid Range: Leu22-His340
Catalog #KR3-HM2L2
Price
Regular price $31500
Regular price Sale price $31500
/
Size
Avantor VWR Fisher Scientific

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Human KIR3DL2 Protein (KR3-HM2L2) Summary

Product Description

 Recombinant Human KIR3DL2 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Leu22-His340.

Product Background

 KIR3DL2 is a member of the killer cell immunoglobulin-like receptor (KIR) family that was initially identified at the surface of natural killer (NK) cells. KIR3DL2, also known as CD158k, is expressed as a disulfide-linked homodimer. Each chain is composed of three immunoglobulin-like domains and a long cytoplasmic tail containing two immunoreceptor tyrosine-based inhibitory motifs.

Human KIR3DL2 Protein (KR3-HM2L2) Specifications/Details

Protein
 KIR3DL2
Synonyms
 NKAT-4; NKAT4; CD158k; CL-5; KIR3DL2; NKAT4A; NKAT4B; p140;CL5
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Leu22-His340
Molecular Weight
 The protein has a predicted MW of 60.92 kDa. Due to glycosylation, the protein migrates to 75-95 kDa, 52-60 kDa and 40-45 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human KIR3DL2, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-KIR3DL2 Antibody, hFc Tag with the EC50 of 60.5ng/ml determined by ELISA.
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human KIR3DL2, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-KIR3DL2 Antibody, hFc Tag with the EC50 of 60.5ng/ml determined by ELISA. Immobilized Human KIR3DL2, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-KIR3DL2 Antibody, hFc Tag with the EC50 of 60.5ng/ml determined by ELISA.
Human KIR3DL2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human KIR3DL2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human KIR3DL2 is greater than 95% as determined by SEC-HPLC. The purity of Human KIR3DL2 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...