SPR binding affinity sensorgram showing dose-dependent interaction between analyte and ligand on biosensor chip.
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 334 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Glu26-Leu322
Catalog #KR3-HM4L3
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Size
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Non-biotinylated Human KIR3DL3 Protein (KR3-HM4L3) Summary

Product Description

 Recombinant Human KIR3DL3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu26-Leu322.

Product Background

 Killer-cell immunoglobulin-like receptor (KIR) 3DL3 is a framework gene present in all human KIR haplotypes.KIR3DL3 has been shown to be constitutively expressed at a low RNA level in peripheral blood mononuclear cell (PBMC) and decidual natural kill (NK) cells.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Non-biotinylated Human KIR3DL3 Protein (KR3-HM4L3) Specifications/Details

Protein
 KIR3DL3
Synonyms
 CD158Z; KIR2DS2; KIR3DL7; KIR44; KIRC1; KIR3DL3
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Glu26-Leu322
Molecular Weight
 The protein has a predicted MW of 35.3 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 334 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His-Avi

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in 20mM Tris, 150mM NaCl, 0.2M L-arginine (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human B7-H7, hFc Tag captured on CM5 Chip via Protein A can bind Human KIR3DL3, His Tag with an affinity constant of 334 nM as determined in SPR assay (Biacore T200). Human B7-H7, hFc Tag captured on CM5 Chip via Protein A can bind Human KIR3DL3, His Tag with an affinity constant of 334 nM as determined in SPR assay (Biacore T200).
Human KIR3DL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human KIR3DL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Human KIR3DL3, His Tag on Anti-His-Biosensor can bind Human B7-H7, hFc Tag with an affinity constant of 24.80 nM as determined in BLI assay. Loaded Human KIR3DL3, His Tag on Anti-His-Biosensor can bind Human B7-H7, hFc Tag with an affinity constant of 24.80 nM as determined in BLI assay.
The purity of Human KIR3DL3 is greater than 95% as determined by SEC-HPLC. The purity of Human KIR3DL3 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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