Human KIR3DL3 Protein (KR3-HM4L3)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 334 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Glu26-Leu322

Catalog: KR3-HM4L3

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Product Description	Recombinant Human KIR3DL3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu26-Leu322.
Product Background	Killer-cell immunoglobulin-like receptor (KIR) 3DL3 is a framework gene present in all human KIR haplotypes.KIR3DL3 has been shown to be constitutively expressed at a low RNA level in peripheral blood mononuclear cell (PBMC) and decidual natural kill (NK) cells.
Product Category	Recombinant Protein

Target	KIR3DL3
Synonyms	CD158Z; KIR2DS2; KIR3DL7; KIR44; KIRC1; KIR3DL3
Gene/Protein ID	Q8N743
Species	Human
Exact Sequence	Glu26-Leu322
Molecular Weight	The protein has a predicted MW of 35.3 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 334 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM Tris, 150mM NaCl, 0.2M L-arginine (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human B7-H7, hFc Tag captured on CM5 Chip via Protein A can bind Human KIR3DL3, His Tag with an affinity constant of 334 nM as determined in SPR assay (Biacore T200).
	The purity of Human KIR3DL3 is greater than 95% as determined by SEC-HPLC.
	Human KIR3DL3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.