Human KLKB1 Protein (KLK-HM1B1)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human KLKB1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-KLKB1 Antibody, hFc Tag with the EC50 of 33.8ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gly20-Ala638

Catalog: KLK-HM1B1

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Product Description	Recombinant Human KLKB1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Ala638.
Product Background	Plasma kallikrein, also known as Fletcher factor or kallikrein B1 (KLKB1), is a serine endopeptidase, like its homologs tissue kallikrein and kallikrein-related peptidases (KLKs). Its physiological role is to catalyze the release of kinins and other vasoactive peptides.
Product Category	Recombinant Protein

Target	KLKB1
Synonyms	Plasma kallikrein; PKK; KLK3; KLKB1; Fletcher factor; Kininogenin
Gene/Protein ID	P03952
Species	Human
Exact Sequence	Gly20-Ala638
Molecular Weight	The protein has a predicted MW of 70.3 kDa. Due to glycosylation, the protein migrates to 72-80 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human KLKB1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-KLKB1 Antibody, hFc Tag with the EC50 of 33.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM NaAc,150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc,150mM NaCl (pH 5.0).
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human KLKB1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-KLKB1 Antibody, hFc Tag with the EC50 of 33.8ng/ml determined by ELISA.
	The purity of Human KLKB1 is greater than 95% as determined by SEC-HPLC.
	Human KLKB1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.