Human KLKB1 Protein (KLK-HM1B1)
Catalog: KLK-HM1B1
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Product Description | Recombinant Human KLKB1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Ala638. |
Product Background |
Plasma kallikrein, also known as Fletcher factor or kallikrein B1 (KLKB1), is a serine endopeptidase, like its homologs tissue kallikrein and kallikrein-related peptidases (KLKs). Its physiological role is to catalyze the release of kinins and other vasoactive peptides. |
Product Category |
Recombinant Protein |
Target |
KLKB1 |
Synonyms | Plasma kallikrein; PKK; KLK3; KLKB1; Fletcher factor; Kininogenin |
Gene/Protein ID |
P03952 |
Species |
Human |
Exact Sequence |
Gly20-Ala638 |
Molecular Weight | The protein has a predicted MW of 70.3 kDa. Due to glycosylation, the protein migrates to 72-80 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Human KLKB1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-KLKB1 Antibody, hFc Tag with the EC50 of 33.8ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in 20mM NaAc,150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc,150mM NaCl (pH 5.0). |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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