Human KLKB1 Protein (KLK-HM1B1)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gly20-Ala638

Catalog: KLK-HM1B1

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Product Description Recombinant Human KLKB1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Ala638.

Product Background

Plasma kallikrein, also known as Fletcher factor or kallikrein B1 (KLKB1), is a serine endopeptidase, like its homologs tissue kallikrein and kallikrein-related peptidases (KLKs). Its physiological role is to catalyze the release of kinins and other vasoactive peptides.

Product Category

Recombinant Protein

Target

KLKB1
Synonyms Plasma kallikrein; PKK; KLK3; KLKB1; Fletcher factor; Kininogenin
Accession

Species

Human

Amino Acid Range

Gly20-Ala638
Molecular Weight The protein has a predicted MW of 70.3 kDa. Due to glycosylation, the protein migrates to 72-80 kDa based on Tris-Bis PAGE result.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 20mM NaAc,150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc,150mM NaCl (pH 5.0).
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC). The specific activity is > 1000 pmol/min/ug.
The purity of Human KLKB1 is greater than 95% as determined by SEC-HPLC.
Human KLKB1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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