Human KLKB1 Protein (pro form), Ultra Low Endotoxin (KLK-HM1B1-UL) Summary
Product Description |
Recombinant Human KLKB1 Protein is expressed from HEK293 with His tag at the C-Terminus. The protein needs to be activated by Thermolysin for an activated form.It contains Gly20-Ala638. |
Product Background |
Plasma kallikrein, also known as Fletcher factor or kallikrein B1 (KLKB1), is a serine endopeptidase, like its homologs tissue kallikrein and kallikrein-related peptidases (KLKs). Its physiological role is to catalyze the release of kinins and other vasoactive peptides. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human KLKB1 Protein (pro form), Ultra Low Endotoxin (KLK-HM1B1-UL) Specifications/Details
Protein |
KLKB1 |
Synonyms |
Plasma kallikrein; PKK; KLK3; KLKB1; Fletcher factor; Kininogenin |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly20-Ala638 |
Molecular Weight |
The protein has a predicted MW of 70.3 kDa. Due to glycosylation, the protein migrates to 72-80 kDa based on Bis-Tris PAGE result. |
Activity |
Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC). The specific activity is > 1000 pmol/min/ug. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM NaAc,150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in 20mM NaAc (pH 5.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
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Human KLKB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human KLKB1 is greater than 95% as determined by SEC-HPLC. |
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