Human KLRG1 Protein (KLR-HM2G1)

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human KLRG1, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-KLRG1 Antibody, hFc Tag with the EC50 of 20.8ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Leu60-Phe195

Catalog: KLR-HM2G1

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Product Description	Recombinant Human KLRG1 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Leu60-Phe195.
Product Background	Immune homeostasis requires the tight, tissue-specific control of the different CD4 Foxp3 regulatory T (Treg) cell populations. The cadherin-binding inhibitory receptor killer cell lectin-like receptor G1 (KLRG1) is expressed by a subpopulation of Treg cells with GATA3 effector phenotype.Lack of KLRG1 on Treg cells conferred on them a competitive advantage in the gut, but not in lymphoid organs. Hence, although absence of KLRG1 is not enough to increase intestinal Treg cells in KLRG1 knockout mice, KLRG1 ligation reduces T-cell receptor signals and the competitive fitness of individual Treg cells in the intestine.
Product Category	Recombinant Protein

Target	KLRG1
Synonyms	KLRG1; CLEC15A; MAFA; MAFAL; 2F1; CLEC15AMAFA-LIKE; MGC13600
Gene/Protein ID	Q96E93-1
Species	Human
Exact Sequence	Leu60-Phe195
Molecular Weight	The protein has a predicted MW of 42.8 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human KLRG1, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-KLRG1 Antibody, hFc Tag with the EC50 of 20.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human KLRG1, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-KLRG1 Antibody, hFc Tag with the EC50 of 20.8ng/ml determined by ELISA.
	The purity of Human KLRG1 is greater than 95% as determined by SEC-HPLC.
	Human KLRG1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.