Product Description |
Recombinant Human KLRG1 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Leu60-Phe195. |
Product Background |
Immune homeostasis requires the tight, tissue-specific control of the different CD4 Foxp3 regulatory T (Treg) cell populations. The cadherin-binding inhibitory receptor killer cell lectin-like receptor G1 (KLRG1) is expressed by a subpopulation of Treg cells with GATA3 effector phenotype.Lack of KLRG1 on Treg cells conferred on them a competitive advantage in the gut, but not in lymphoid organs. Hence, although absence of KLRG1 is not enough to increase intestinal Treg cells in KLRG1 knockout mice, KLRG1 ligation reduces T-cell receptor signals and the competitive fitness of individual Treg cells in the intestine. |
Product Category |
Recombinant Protein |
Target |
KLRG1 |
Synonyms |
KLRG1; CLEC15A; MAFA; MAFAL; 2F1; CLEC15AMAFA-LIKE; MGC13600 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu60-Phe195 |
Molecular Weight |
The protein has a predicted MW of 42.8 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human KLRG1, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-KLRG1 Antibody, hFc Tag with the EC50 of 24.9ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human KLRG1, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-KLRG1 Antibody, hFc Tag with the EC50 of 24.9ng/ml determined by ELISA. |
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Human KLRG1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human KLRG1 is greater than 95% as determined by SEC-HPLC. |
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