Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Tetramer Protein (MHC-HM420T)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) TCR at 1ug/ml (100ul/well) on the plate. Dose response curve for Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Tetramer, His Tag with the EC50 of 51.5ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly25-Thr305(HLA-A*11:01),Ile21-Met119(B2M)and VVVGADGVGK peptide

Catalog: MHC-HM420T

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Product Description Recombinant Human KRAS G12D(HLA-A*11:01) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*11:01),Ile21-Met119(B2M) and VVVGADGVGK peptide.

Product Background

Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. The virtual screening approach to discover novel KRAS inhibitors and synthetic lethality interactors of KRAS are discussed in detail.

Product Category

Recombinant Protein

Target

HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Tetramer
Synonyms MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD
Accession
MHC Form Tetramer
Allele HLA-A*11:01

Species

Human

Amino Acid Range

Gly25-Thr305(HLA-A*11:01),Ile21-Met119(B2M)and VVVGADGVGK peptide
Molecular Weight The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

Activity

Immobilized HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) TCR at 1ug/ml (100ul/well) on the plate. Dose response curve for Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Tetramer, His Tag with the EC50 of 51.5ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) TCR at 1ug/ml (100ul/well) on the plate. Dose response curve for Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Tetramer, His Tag with the EC50 of 51.5ng/ml determined by ELISA.
The purity of Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Tetramer is greater than 95% as determined by SEC-HPLC.
Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.

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