Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Tetramer Protein (MHC-HM420T)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) TCR at 1ug/ml (100ul/well) on the plate. Dose response curve for Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Tetramer, His Tag with the EC50 of 49.9ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gly25-Thr305(HLA-A*11:01),Ile21-Met119(B2M)and VVVGADGVGK peptide

Catalog: MHC-HM420T

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Product Description Recombinant Human KRAS G12D(HLA-A*11:01) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*11:01),Ile21-Met119(B2M) and VVVGADGVGK peptide.
Product Category Recombinant Protein
Target HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Tetramer
Synonyms MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD
Gene/Protein ID AAV53343.1(HLA-A*11:01)&P61769(B2M)&VVVGADGVGK
Species Human
Exact Sequence Gly25-Thr305(HLA-A*11:01),Ile21-Met119(B2M)and VVVGADGVGK peptide
Molecular Weight The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
Activity Immobilized HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) TCR at 1ug/ml (100ul/well) on the plate. Dose response curve for Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Tetramer, His Tag with the EC50 of 49.9ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) TCR at 1ug/ml (100ul/well) on the plate. Dose response curve for Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Tetramer, His Tag with the EC50 of 49.9ng/ml determined by ELISA.
The purity of Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Tetramer is greater than 95% as determined by SEC-HPLC.
Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.

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