Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Tetramer Protein (MHC-HM420T)
Catalog: MHC-HM420T
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| Product Description | Recombinant Human KRAS G12D(HLA-A*11:01) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*11:01),Ile21-Met119(B2M) and VVVGADGVGK peptide. |
| Product Category | Recombinant Protein |
| Target | HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Tetramer |
| Synonyms | MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD |
| Gene/Protein ID | AAV53343.1(HLA-A*11:01)&P61769(B2M)&VVVGADGVGK |
| Species | Human |
| Exact Sequence | Gly25-Thr305(HLA-A*11:01),Ile21-Met119(B2M)and VVVGADGVGK peptide |
| Molecular Weight | The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. |
| Activity | Immobilized HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) TCR at 1ug/ml (100ul/well) on the plate. Dose response curve for Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Tetramer, His Tag with the EC50 of 49.9ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) TCR at 1ug/ml (100ul/well) on the plate. Dose response curve for Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Tetramer, His Tag with the EC50 of 49.9ng/ml determined by ELISA. |
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The purity of Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Tetramer is greater than 95% as determined by SEC-HPLC. |
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Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%. |
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