Name: Human HLA-A*03:01&B2M&KRAS WT (VVVGAGGVGK) Monomer Protein (MHC-HM423)
Brand: KactusBio
SKU: MHC-HM423-100UG
Price: 310.0 USD
Availability: InStock

Human HLA-A*03:01&B2M&KRAS WT (VVVGAGGVGK) Monomer Protein (MHC-HM423)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Amino Acid Range: Gly25-Thr305(HLA-A*03:01), Ile21-Met119(B2M) and VVVGAGGVGK peptide

Catalog #MHC-HM423

Size

100UG

500UG

1MG

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Product Description	Recombinant Human KRAS WT(HLA-A03:01) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A03:01), Ile21-Met119(B2M) and VVVGAGGVGK peptide.
Product Background	Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target.
Product Category	Recombinant Protein

Target	HLA-A*03:01&B2M&KRAS WT (VVVGAGGVGK) Monomer
Synonyms	MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD
MHC Form	Monomer
Allele	HLA-A*03:01
Accession	NP_002107.3(HLA-A*03:01)&P61769(B2M)&VVVGAGGVGK
Species	Human
Biotinylated	no
Amino Acid Range	Gly25-Thr305(HLA-A*03:01), Ile21-Met119(B2M) and VVVGAGGVGK peptide
Molecular Weight	The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Bis-Tris PAGE result.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human HLA-A*03:01&B2M&KRAS WT (VVVGAGGVGK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human HLA-A*03:01&B2M&KRAS WT (VVVGAGGVGK) Monomer is greater than 95% as determined by SEC-HPLC.

Product Resources

Learn More: MHC Collection Learn More: Peptide-ready MHC Protocol: MHC Staining Case Study: Peptide-ready MHC for TCR Staining Poster: Engineering MHCs as Versatile Reagents

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