Human Kremen-1 Protein (KRE-HM201) Summary
Product Description |
Recombinant Human Kremen-1 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Arg21-Thr392. |
Product Background |
KREMEN1 (KRM1) has been identified as a functional receptor for Coxsackievirus A10 (CV-A10), a causative agent of hand-foot-and-mouth disease (HFMD), which poses a great threat to infants globally. KRM1 selectively binds to the mature viral particle above the canyon of the viral protein 1 (VP1) subunit and contacts across two adjacent asymmetry units. The key residues for receptor binding are conserved among most KRM1-dependent enteroviruses, suggesting a uniform mechanism for receptor binding. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human Kremen-1 Protein (KRE-HM201) Specifications/Details
Protein |
Kremen-1 |
Synonyms |
Kremen protein 1; KREMEN; KRM1; KREMEN1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Arg21-Thr392 |
Molecular Weight |
The protein has a predicted MW of 67.6 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-hFc (IgG1) |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human Kremen-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human Kremen-1 is greater than 95% as determined by SEC-HPLC. |
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