Human L1CAM Protein (LAM-HM101)
Catalog: LAM-HM101
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Product Description | Recombinant Human L1CAM Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile20-Glu1120. |
Product Category | Recombinant Protein |
Target | L1CAM |
Synonyms | N-CAM-L1; NCAM-L1; CD171; L1CAM; CAML1; MIC5; CALL; CHL1; CHL-1; FLJ44930; L1CAM-2; MGC132578; HSAS; HSAS1; MASA; S10; SPG1 |
Gene/Protein ID | P32004-1 |
Species | Human |
Exact Sequence | Ile20-Glu1120 |
Molecular Weight | The protein has a predicted MW of 124.6 kDa. Due to glycosylation, the protein migrates to 150-180 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human L1CAM, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-L1CAM Antibody, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA. See testing image for detail. |
Product Tag | C-His |
Express System | HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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