Human L1CAM Protein (LAM-HM101)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human L1CAM, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-L1CAM Antibody, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ile20-Glu1120

Catalog: LAM-HM101

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Product Description Recombinant Human L1CAM Protein is expressed from Expi293 with His tag at the C-terminal.It contains Ile20-Glu1120.
Product Category Recombinant Protein
Target L1CAM
Synonyms N-CAM-L1; NCAM-L1; CD171; L1CAM; CAML1; MIC5; CALL; CHL1; CHL-1; FLJ44930; L1CAM-2; MGC132578; HSAS; HSAS1; MASA; S10; SPG1
Gene/Protein ID P32004-1
Species Human
Exact Sequence Ile20-Glu1120
Molecular Weight The protein has a predicted MW of 124.6 kDa. Due to glycosylation, the protein migrates to 150-180 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human L1CAM, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-L1CAM Antibody, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human L1CAM, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-L1CAM Antibody, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA.
The purity of Human L1CAM is greater than 95% as determined by SEC-HPLC.
Human L1CAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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