Human LAG3/CD223 Protein (LAG-HM131)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LAG3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LAG3 Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Exact Sequence: Leu23-Gly434

Catalog: LAG-HM131

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Product Description Recombinant Human LAG3/CD223 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu23-Gly434.
Product Category Recombinant Protein
Target LAG3/CD223
Synonyms CD223; FDC; LAG3; LAG-3; sLAG-3
Gene/Protein ID P18627-1
Species Human
Exact Sequence Leu23-Gly434
Molecular Weight The protein has a predicted MW of 45.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human LAG3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LAG3 Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag C-His
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human LAG3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LAG3 Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA (QC Test).
Immobilized Human LAG3, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human FGL1, hFc Tag with the EC50 of 0.91ug/ml determined by ELISA.
The purity of Human LAG3 is greater than 95% as determined by SEC-HPLC.
Human LAG3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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