Name: Human LAG3/CD223 Protein (LAG-HM131)
Brand: KactusBio
SKU: LAG-HM131-100UG
Price: 285.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human LAG3, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human FGL1, hFc Tag with the EC50 of 0.28ug/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Leu23-Gly434

Catalog #LAG-HM131

Size

100UG

500UG

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Product Description	Recombinant Human LAG3/CD223 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu23-Gly434.
Product Background	LAG-3, is a protein which in humans is encoded by the LAG3 gene, which is a cell surface molecule with diverse biologic effects on T cell function. It is an immune checkpoint receptor and as such is the target of various drug development programs by pharmaceutical companies seeking to develop new treatments for cancer and autoimmune disorders.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	LAG3/CD223
Synonyms	CD223; FDC; LAG3; LAG-3; sLAG-3
Accession	P18627-1
Species	Human
Biotinylated	no
Amino Acid Range	Leu23-Gly434
Molecular Weight	The protein has a predicted MW of 45.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human LAG3, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human FGL1, hFc Tag with the EC50 of 0.28ug/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human LAG3, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human FGL1, hFc Tag with the EC50 of 0.28ug/ml determined by ELISA (QC Test).
	Immobilized Human LAG3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LAG3 Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA.
	Human LAG3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human LAG3 is greater than 95% as determined by SEC-HPLC.