Human LAG3/CD223 Protein (LAG-HM231)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human FGL1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human LAG3, hFc Tag with the EC50 of 75.1ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Leu23-Leu450

Catalog: LAG-HM231

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Product Description	Recombinant Human LAG3/CD223 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu23-Leu450.
Product Background	LAG-3, is a protein which in humans is encoded by the LAG3 gene, which is a cell surface molecule with diverse biologic effects on T cell function. It is an immune checkpoint receptor and as such is the target of various drug development programs by pharmaceutical companies seeking to develop new treatments for cancer and autoimmune disorders.
Product Category	Recombinant Protein

Target	LAG3/CD223
Synonyms	CD223; FDC; LAG3; LAG-3; sLAG-3
Gene/Protein ID	P18627-1
Species	Human
Exact Sequence	Leu23-Leu450
Molecular Weight	The protein has a predicted MW of 72.4 kDa. Due to glycosylation, the protein migrates to 75-80 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human FGL1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human LAG3, hFc Tag with the EC50 of 75.1ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human FGL1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human LAG3, hFc Tag with the EC50 of 75.1ng/ml determined by ELISA.
	Human LAG3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%