• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human Latent GDF-11, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GDF11 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA.
  • Amino Acid Range: Ala25-Ser407
Catalog #GDF-HM111
Price
Regular price $49500
Regular price Sale price $49500
/
Size
Avantor VWR Fisher Scientific

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Human Latent GDF-11 Protein (pro&latent) (GDF-HM111) Summary

Product Description

 Recombinant Human Latent GDF-11 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ala25-Ser407.

Product Background

 Growth differentiation factor-11 (GDF-11), also known as bone morphogenetic protein-11, belongs to the transforming growth factor-beta superfamily. GDF-11 is highly related to MSTN, plays multiple roles during embryonic development, including regulating development of the axial skeleton, kidneys, nervous system, and pancreas.

Human Latent GDF-11 Protein (pro&latent) (GDF-HM111) Specifications/Details

Protein
 GDF-11
Synonyms
 GDF11; BMP-11;;BMP11;Growth differentiation factor-11; Growth differentiation factor 11; Bone morphogenetic protein-11; Bone morphogenetic protein 11
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala25-Ser407
Molecular Weight
 The protein has a predicted MW of 44.14 kDa. Due to glycosylation, the protein migrates to 15-20 kDa, 40-45 kDa and 50-60 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Latent GDF-11, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GDF11 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human Latent GDF-11, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GDF11 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA. Immobilized Human Latent GDF-11, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GDF11 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA.
Immobilized Human Latent GDF-11, His Tag at 2ug/ml(100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA. Immobilized Human Latent GDF-11, His Tag at 2ug/ml(100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA.
Human Latent GDF-11 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Latent GDF-11 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Anti-GDF-11 Antibody, hFc Tag on ProA-Biosensor can bind Human Latent GDF-11, His Tag with an affinity constant of 0.15 nM as determined in BLI assay. Loaded Anti-GDF-11 Antibody, hFc Tag on ProA-Biosensor can bind Human Latent GDF-11, His Tag with an affinity constant of 0.15 nM as determined in BLI assay.
Other Available Proteins:
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