Name: Human Latent GDF-8 Protein (GDF-HM108)
Brand: KactusBio
SKU: GDF-HM108-100UG
Price: 363.0 USD
Availability: InStock

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Latent GDF-8, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GDF8 Antibody, hFc Tag with the EC50 of 22.8 ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Asn24-Ser375

Catalog #GDF-HM108

Size

100UG

500UG

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Product Description	Recombinant Human Latent GDF-8 Protein is expressed from HEK293 with His tag at the N-terminus.It contains Asn24-Ser375.
Product Background	Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain. GDF8, like numerous TGF-β family members, is a disulfidelinked dimer that is synthesized as a precursor protein which requires cleavage by a furin-like protease to yield an N-terminal prodomain and a C-terminal mature, signaling domain.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	Latent GDF-8
Synonyms	Myostatin; GDF8; Latent GDF8; Mstn
Accession	O14793
Species	Human
Biotinylated	no
Amino Acid Range	Asn24-Ser375
Molecular Weight	The protein has a predicted MW of 41.19 kDa. Due to glycosylation, the protein migrates to 14 kDa, 38-40 kDa and 45-52 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human Latent GDF-8, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GDF8 Antibody, hFc Tag with the EC50 of 22.8 ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS, 200mM L-Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human Latent GDF-8, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GDF8 Antibody, hFc Tag with the EC50 of 22.8 ng/ml determined by ELISA.
	Immobilized Human Latent GDF-8, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag with the EC50 of 33.7ng/ml determined by ELISA.
	Human Latent GDF-8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Anti-GDF8 Antibody, hFc Tag on ProA-Biosensor can bind Human Latent GDF-8, His Tag with an affinity constant of 0.30 µM as determined in BLI assay (Gator® Prime).
	The purity of Human Latent GDF-8 is greater than 95% as determined by SEC-HPLC.