Human Latent GDF-8/Myostatin HPLC data showing high purity protein | KACTUS
Human Latent GDF-8/Myostatin Protein ELISA bioactivity data showing binding to anti-GDF-8 antibody | KACTUS
Tris-Bis gel showing greater than 95% purity of Human Latent GDF-8/Myostatin protein | KACTUS
Human Latent GDF-8/Myostatin HPLC data showing high purity protein | KACTUS
Human Latent GDF-8/Myostatin Protein ELISA bioactivity data showing binding to anti-GDF-8 antibody | KACTUS
Tris-Bis gel showing greater than 95% purity of Human Latent GDF-8/Myostatin protein | KACTUS
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Human Latent GDF-8, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GDF8 Antibody, hFc Tag with the EC50 of 22.8 ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Asn24-Ser375
Catalog #GDF-HM108
/
Size

Order now, ships by:

Product Description
 Recombinant Human Latent GDF-8 Protein is expressed from HEK293 with His tag at the N-terminus.It contains Asn24-Ser375.
Product Background
 Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain. GDF8, like numerous TGF-β family members, is a disulfidelinked dimer that is synthesized as a precursor protein which requires cleavage by a furin-like protease to yield an N-terminal prodomain and a C-terminal mature, signaling domain.
Product Category
 Recombinant Protein
Target
 Latent GDF-8
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Asn24-Ser375
Molecular Weight
 The protein has a predicted MW of 41.19 kDa. Due to glycosylation, the protein migrates to 35-40 kDa and 45-55 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Latent GDF-8, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GDF8 Antibody, hFc Tag with the EC50 of 22.8 ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
 N-His
Express System
 HEK293
Purity
 >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Latent GDF-8, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GDF8 Antibody, hFc Tag with the EC50 of 22.8 ng/ml determined by ELISA (QC Test).
Human Latent GDF-8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Latent GDF-8 is greater than 95% as determined by SEC-HPLC.

Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Contact Us

support@kactusbio.us

Recently viewed