Human Latent GDF-8 Protein (GDF-HM128) Summary
Product Description |
Recombinant Human Latent GDF-8 Protein is expressed from HEK293 with His tag at the N-terminus.It contains Asn24-Ser375. |
Product Background |
Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain. GDF8, like numerous TGF-β family members, is a disulfidelinked dimer that is synthesized as a precursor protein which requires cleavage by a furin-like protease to yield an N-terminal prodomain and a C-terminal mature, signaling domain. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human Latent GDF-8 Protein (GDF-HM128) Specifications/Details
Protein |
GDF-8 |
Synonyms |
Myostatin; GDF8; Latent GDF8; Mstn |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asn24-Ser375 |
Molecular Weight |
The protein has a predicted MW of 41.19 kDa. Due to glycosylation, the protein migrates to 14 kDa and 32-38 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Latent GDF-8, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Apitegromab, hFc Tag with the EC50 of 23.1ng/ml determined by ELISA. |
Product Tag |
N-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human Latent GDF-8, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Apitegromab, hFc Tag with the EC50 of 23.1ng/ml determined by ELISA. |
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Immobilized Human Latent GDF-8, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA. |
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Human Latent GDF-8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human Latent GDF-8 is greater than 90% as determined by SEC-HPLC. |
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