Human Latent TGF beta 1/TGFB1 Protein (TG1-HM401)

  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human Latent TGF beta 1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 86.1ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu30-Ser390(C33S)

Catalog: TG1-HM401

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Product Description Recombinant Human Latent TGF beta 1/TGFB1 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Leu30-Ser390(C33S).
Product Category Recombinant Protein
Target Latent TGF beta 1/TGFB1
Synonyms CEDLAP; DPD1; TGF beta1; TGFB; TGFB1; TGFbeta; TGF-beta-1
Gene/Protein ID P01137
Species Human
Exact Sequence Leu30-Ser390(C33S)
Molecular Weight The protein has a predicted MW of 44.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa(LAP) / 13-15 kDa(Mature TGF Beta 1) kDa based on Tris-Bis PAGE result.
Activity Immobilized Human Latent TGF beta 1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 86.1ng/ml determined by ELISA. See testing image for detail.
Product Tag N-His
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Latent TGF beta 1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 86.1ng/ml determined by ELISA.
Immobilized Human Latent TGF beta 1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB8, His Tag with the EC50 of 6.6ug/ml determined by ELISA.
Immobilized Human Latent TGF beta 1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, His Tag with the EC50 of 1.2ug/ml determined by ELISA.
Human Latent TGF beta 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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