Name: Human Latent TGF beta 1/TGFB1 Protein (TG1-HM401)
Brand: KactusBio
SKU: TG1-HM401-100UG
Price: 445.5 USD
Availability: InStock

ELISA binding curve showing the dose-dependent interaction between Human Latent TGF beta 1, His Tag, and Human TGF-beta RII, hFc Tag. The plot displays mean absorbance (OD450) versus the log concentration of the receptor (µg/ml), indicating a sigmoidal response.

Human Latent TGF beta 1/TGFB1 Protein (TG1-HM401)

Immobilized Human Latent TGF beta 1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, His Tag with the EC50 of 2.2ug/ml determined by ELISA (QC Test).

Immobilized Human Latent TGF beta 1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 86.1ng/ml determined by ELISA.

Immobilized Human Latent TGF beta 1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB8, His Tag with the EC50 of 9.62ug/ml determined by ELISA.

Human Latent TGF beta 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human Latent TGF beta 1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, His Tag with the EC50 of 2.2ug/ml determined by ELISA (QC Test).
Amino Acid Range: Leu30-Ser390(C33S)

Catalog #TG1-HM401

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Human Latent TGF beta 1/TGFB1 Protein (TG1-HM401) Summary

Product Description	Recombinant Human Latent TGF beta 1/TGFB1 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Leu30-Ser390 (C33S).
Product Background	Latent TGF beta 1 -cDNA encodes a 390 amino acid precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1. Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human Latent TGF beta 1/TGFB1 Protein (TG1-HM401) Specifications/Details

Protein	Latent TGF beta 1/TGFB1
Synonyms	CEDLAP; DPD1; TGF beta1; TGFB; TGFB1; TGFbeta; TGF-beta-1; TGF β1; TGFβ; TGF-β-1; TGF-beta 1
Accession	P01137
Species	Human
Biotinylated	no
Amino Acid Range	Leu30-Ser390(C33S)
Molecular Weight	The protein has a predicted MW of 44.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa (LAP) / 13-15 kDa (Mature TGF Beta 1) based on Bis-Tris PAGE result.
Activity	Immobilized Human Latent TGF beta 1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, His Tag with the EC50 of 2.2ug/ml determined by ELISA (QC Test).
Product Tag	N-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human Latent TGF beta 1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, His Tag with the EC50 of 2.2ug/ml determined by ELISA (QC Test).
	Immobilized Human Latent TGF beta 1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 86.1ng/ml determined by ELISA.
	Immobilized Human Latent TGF beta 1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB8, His Tag with the EC50 of 9.62ug/ml determined by ELISA.
	Human Latent TGF beta 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.