Product Description |
Recombinant Human Latent TGF beta 3 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Leu24-Ser412. |
Product Background |
Transforming growth factor-betas (TGF-βs) are multifunctional cytokines that have been implicated in the regulation of a broad range of biological processes, including cell proliferation, cell survival, and cell differentiation. And transforming growth factor beta3 (TGFbeta3) is a key protein involved in scar-free healing observed in embryos. |
Product Category |
Recombinant Protein |
Target |
Latent TGF beta 3 |
Synonyms |
TGFB3; ARVD; TGF-beta3; TGF-β; TGFβ3; TGFβ |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu24-Ser412 |
Molecular Weight |
The protein has a predicted MW of 45.9 kDa. Due to glycosylation, the protein migrates to 46-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Latent TGF beta 3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 5.7ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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