Human Latent TGF beta 3 Protein (TGF-HM103)

  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Latent TGF beta 3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 12.1ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu24-Ser412

Catalog: TGF-HM103

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Product Description Recombinant Human Latent TGF beta 3 Protein is expressed from HEK293 with His tag at the N-terminal.It contains Leu24-Ser412.
Product Category Recombinant Protein
Target Latent TGF beta 3
Gene/Protein ID P10600-1
Species Human
Exact Sequence Leu24-Ser412
Molecular Weight The protein has a predicted MW of 45.9 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 46-50 kDa and 55-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human Latent TGF beta 3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 12.1ng/ml determined by ELISA. See testing image for detail.
Product Tag N-His
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in 20mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Latent TGF beta 3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 12.1ng/ml determined by ELISA.
The purity of Human Latent TGF beta 3 is greater than 95% as determined by SEC-HPLC.
Human Latent TGF beta 3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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