Human Latent TGF beta 3 Protein (TGF-HM103)

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human Latent TGF beta 3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 14.7ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Leu24-Ser412

Catalog: TGF-HM103

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Product Description	Recombinant Human Latent TGF beta 3 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Leu24-Ser412.
Product Background	Transforming growth factor-betas (TGF-βs) are multifunctional cytokines that have been implicated in the regulation of a broad range of biological processes, including cell proliferation, cell survival, and cell differentiation. And transforming growth factor beta3 (TGFbeta3) is a key protein involved in scar-free healing observed in embryos.
Product Category	Recombinant Protein

Target	Latent TGF beta 3
Gene/Protein ID	P10600-1
Species	Human
Exact Sequence	Leu24-Ser412
Molecular Weight	The protein has a predicted MW of 45.9 kDa. Due to glycosylation, the protein migrates to 46-60 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human Latent TGF beta 3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 14.7ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human Latent TGF beta 3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 14.7ng/ml determined by ELISA.
	Human Latent TGF beta 3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.