Human Latexin-Protein (LAT-HE101)

Express System: E.coli
Product Tag: N-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Measured by its ability to inhibit carboxypeptidase A1 cleavage of the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB). The IC50-value is < 1 nM.
Amino Acid Range: Glu2-Glu222

Catalog #LAT-HE101

Price

$330⁰⁰

Size

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Human Latexin-Protein (LAT-HE101) Summary

Product Description

Recombinant Human Latexin-Protein is expressed from E.coli with His tag at the N-Terminus.It contains Glu2-Glu222.

Product Background

Latexin, the endogenous protein inhibitor of the A/B subfamily of metallocarboxypeptidases, is expressed in small nociceptive neurons in sensory ganglia and in a subset of neurons in the telencephalon. Recently, the latexin (Lxn) gene was identified as a potential tumor suppressor in several types of solid tumors and lymphoma, and Lxn expression was found to be absent or downregulated in leukemic cells.

Human Latexin-Protein (LAT-HE101) Specifications/Details

Protein	Latexin
Synonyms	ECI; MUM; TCI; LXN
Accession	AAH05346
Species	Human
Biotinylated	no
Amino Acid Range	Glu2-Glu222
Molecular Weight	The protein has a predicted MW of 27.16 kDa. The protein migrates to 30-35 kDa based on Bis-Tris PAGE result.
Activity	Measured by its ability to inhibit carboxypeptidase A1 cleavage of the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB). The IC50-value is < 1 nM.
Product Tag	N-His
Fluorophore	Unconjugated
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human Latexin-on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human Latexin is greater than 95% as determined by SEC-HPLC.