Human LAYN Protein (LAY-HM101)
Human LAYN Protein (LAY-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Ala22-Leu235
Catalog #LAY-HM101
Regular price $297.00
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Human LAYN Protein (LAY-HM101) Summary

Product Description

Recombinant Human LAYN Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala22-Leu235.

Product Background

Layilin (LAYN) is a critical gene that regulates T cell function. LAYN is correlated with prognosis and immune infiltrating levels of, including those of CD8 T cells, CD4 T cells, macrophages, neutrophils, and DCs in multiple cancers, especially in colon and gastric cancer patients. In addition, LAYN expression potentially contributes to regulation of tumor-associated macrophages (TAMs), DCs, T cell exhaustion and Tregs in colon and gastric cancer.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human LAYN Protein (LAY-HM101) Specifications/Details

Protein
LAYN
Synonyms
Layilin; FLJ30977; FLJ31092; LAYN; LYAN
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ala22-Leu235
Molecular Weight
The protein has a predicted MW of 25.4 kDa. Due to glycosylation, the protein migrates to 35-42 kDa based on Bis-Tris PAGE result.
Product Tag
C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human LAYN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human LAYN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human LAYN is greater than 95% as determined by SEC-HPLC. The purity of Human LAYN is greater than 95% as determined by SEC-HPLC.
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