Name: Human LCAT Protein (LAT-HM101)
Brand: KactusBio
SKU: LAT-HM101-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human LCAT, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LCAT Antibody, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Phe25-Glu440

Catalog #LAT-HM101

Size

100UG

500UG

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Product Description	Recombinant Human LCAT Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe25-Glu440.
Product Background	Lecithin:cholesterol acyltransferase (LCAT) is the only enzyme capable of esterifying cholesterol in plasma, thus determining the maturation of high-density lipoproteins. Because it maintains an unesterified cholesterol gradient between peripheral cells and extracellular acceptors, for a long time, LCAT has been considered as a key enzyme in reverse cholesterol transport.
Product Category	Recombinant Protein / Antibody discovery / Antibody discover \| Others

Protein	LCAT
Synonyms	PAF acetylhydrolase; LCAT
Accession	P04180
Species	Human
Biotinylated	no
Amino Acid Range	Phe25-Glu440
Molecular Weight	The protein has a predicted MW of 48.2 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human LCAT, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LCAT Antibody, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human LCAT, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LCAT Antibody, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA.
	Human LCAT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.