Human LCAT Protein (LAT-HM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human LCAT, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LCAT Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Phe25-Glu440

Catalog: LAT-HM101

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Product Description Recombinant Human LCAT Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe25-Glu440.

Product Background

Lecithin:cholesterol acyltransferase (LCAT) is the only enzyme capable of esterifying cholesterol in plasma, thus determining the maturation of high-density lipoproteins. Because it maintains an unesterified cholesterol gradient between peripheral cells and extracellular acceptors, for a long time, LCAT has been considered as a key enzyme in reverse cholesterol transport.

Product Category

Recombinant Protein

Target

LCAT
Synonyms PAF acetylhydrolase; LCAT
Accession

Species

Human

Amino Acid Range

Phe25-Glu440
Molecular Weight The protein has a predicted MW of 48.2 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human LCAT, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LCAT Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human LCAT, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LCAT Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA.
Human LCAT on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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