Human LCAT Protein (LAT-HM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human LCAT, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LCAT Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Phe25-Glu440

Catalog: LAT-HM101

Order now get it on

Bulk Quote | Test Sample

Product Description	Recombinant Human LCAT Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe25-Glu440.
Product Background	Lecithin:cholesterol acyltransferase (LCAT) is the only enzyme capable of esterifying cholesterol in plasma, thus determining the maturation of high-density lipoproteins. Because it maintains an unesterified cholesterol gradient between peripheral cells and extracellular acceptors, for a long time, LCAT has been considered as a key enzyme in reverse cholesterol transport.
Product Category	Recombinant Protein

Target	LCAT
Synonyms	PAF acetylhydrolase; LCAT
Gene/Protein ID	P04180
Species	Human
Exact Sequence	Phe25-Glu440
Molecular Weight	The protein has a predicted MW of 48.2 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human LCAT, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LCAT Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human LCAT, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LCAT Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA.
	Human LCAT on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.