• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Amino Acid Range: Phe25-Glu440
Catalog #LAT-HM201
Price
Regular price $000
Regular price Sale price $000
/
Size
Avantor VWR Fisher Scientific

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Human LCAT Protein (LAT-HM201) Summary

Product Description

 Recombinant Human LCAT Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Phe25-Glu440.

Product Background

 Lecithin:cholesterol acyltransferase (LCAT) is the only enzyme capable of esterifying cholesterol in plasma, thus determining the maturation of high-density lipoproteins. Because it maintains an unesterified cholesterol gradient between peripheral cells and extracellular acceptors, for a long time, LCAT has been considered as a key enzyme in reverse cholesterol transport.

Human LCAT Protein (LAT-HM201) Specifications/Details

Protein
 LCAT
Synonyms
 PAF acetylhydrolase; LCAT
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Phe25-Glu440
Molecular Weight
 The protein has a predicted MW of 73.02 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result.
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human LCAT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human LCAT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human LCAT is greater than 90% as determined by SEC-HPLC. The purity of Human LCAT is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...