• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 85% as determined by HPLC
  • Activity: Immobilized Human LDLR, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human PCSK9 (D374Y), His Tag with the EC50 of 32.1ng/ml determined by ELISA. The affinity constant of 11.25 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Ala22-Arg788
Catalog #LDL-HM201
Price
Regular price $27000
Regular price Sale price $27000
/
Size
Avantor VWR Fisher Scientific

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Human LDLR Protein (LDL-HM201) Summary

Product Description

 Recombinant Human LDLR Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Ala22-Arg788.

Product Background

 The low density lipoprotein receptor (LDLR) is the founding member of the LDL R family of widely expressed cell surface scavenger receptors. It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles.

Human LDLR Protein (LDL-HM201) Specifications/Details

Protein
 LDLR
Synonyms
 LDLR; FHC; LDL R; LDL receptor; LDLCQ2; FH
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala22-Arg788
Molecular Weight
 The protein has a predicted MW of 110.72 kDa. Due to glycosylation, the protein migrates to 140-180 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human LDLR, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human PCSK9 (D374Y), His Tag with the EC50 of 32.1ng/ml determined by ELISA. The affinity constant of 11.25 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 85% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human LDLR, hFc Tag captured on CM5 Chip via Protein A can bind Human PCSK9, His Tag (Cat. PCS-HM190) with an affinity constant of 11.25 nM as determined in SPR assay (Biacore T200). Human LDLR, hFc Tag captured on CM5 Chip via Protein A can bind Human PCSK9, His Tag (Cat. PCS-HM190) with an affinity constant of 11.25 nM as determined in SPR assay (Biacore T200).
Immobilized Human LDLR, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human PCSK9 (D374Y), His Tag with the EC50 of 32.1ng/ml determined by ELISA. Immobilized Human LDLR, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human PCSK9 (D374Y), His Tag with the EC50 of 32.1ng/ml determined by ELISA.
Human LDLR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human LDLR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human LDLR is greater than 85% as determined by SEC-HPLC. The purity of Human LDLR is greater than 85% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...