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Non-biotinylated Human LDLR Protein, Ultra Low Endotoxin (LDL-HM401-UL) Summary
Product Description |
Recombinant Human LDLR Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala22-Arg788. |
Product Background |
The low density lipoprotein receptor (LDLR) is the founding member of the LDL R family of widely expressed cell surface scavenger receptors. It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Non-biotinylated Human LDLR Protein, Ultra Low Endotoxin (LDL-HM401-UL) Specifications/Details
Protein |
LDLR |
Synonyms |
LDLR; FHC; LDL R; LDL receptor; LDLCQ2; FH |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala22-Arg788 |
Molecular Weight |
The protein has a predicted MW of 87.6 kDa, Due to glycosylation, the protein migrates to 110-130 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.35 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
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Human LDLR, His Tag immobilized on CM5 Chip can bind Human PCSK9, His Tag with an affinity constant of 0.35 nM as determined in SPR assay (Biacore T200). |
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Human LDLR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human LDLR is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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