Name: Human LGR-4 Protein (LGR-HM104)
Brand: KactusBio
SKU: LGR-HM104-100UG
Price: 346.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human LGR-4, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LGR-4 Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. The affinity constant of 0.440 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Leu28–Leu396

Catalog #LGR-HM104

Size

100UG

500UG

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Product Description	Recombinant Human LGR-4 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Leu28–Leu396.
Product Background	Leucine-rich repeat-containing G protein-coupled receptor (LGR)-4 is a G protein-coupled receptor (GPCR) with a seven-transmembrane domain structure. LGRs are evolutionally and structurally phylogenetic, classified into three subgroups and are members of the so-called orphan receptors.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	LGR-4
Synonyms	LGR4; GPR48
Accession	Q9BXB1-1
Species	Human
Biotinylated	no
Amino Acid Range	Leu28–Leu396
Molecular Weight	The protein has a predicted MW of 50.20 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human LGR-4, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LGR-4 Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. The affinity constant of 0.440 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human R Spondin 3, His Tag immobilized on CM5 Chip can bind Human LGR4, His Tag with an affinity constant of 0.440 uM as determined in SPR assay (Biacore T200).
	Immobilized Human LGR-4, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LGR-4 Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA.
	Human LGR-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human LGR-4 is greater than 95% as determined by SEC-HPLC.