Human LGR-4 Protein (LGR-HM104)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.440 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Leu28–Leu396

Catalog: LGR-HM104

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Product Description	Recombinant Human LGR-4 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Leu28–Leu396.
Product Background	Leucine-rich repeat-containing G protein-coupled receptor (LGR)-4 is a G protein-coupled receptor (GPCR) with a seven-transmembrane domain structure. LGRs are evolutionally and structurally phylogenetic, classified into three subgroups and are members of the so-called orphan receptors.
Product Category	Recombinant Protein

Target	LGR-4
Accession	Q9BXB1-1
Species	Human
Amino Acid Range	Leu28–Leu396
Molecular Weight	The protein has a predicted MW of 50.20 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 0.440 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human R Spondin 3, His Tag immobilized on CM5 Chip can bind Human LGR4, His Tag with an affinity constant of 0.440 uM as determined in SPR assay (Biacore T200).
	The purity of Human LGR-4 is greater than 95% as determined by SEC-HPLC.
	Human LGR-4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.