Product Description |
Recombinant Human LGR-4 Protein-Nanodisc is expressed from HEK293 with His tag at the C-terminus.It contains Ala25-Asp951. |
Product Background |
Leucine-rich repeat-containing G protein-coupled receptor (LGR)-4 is a G protein-coupled receptor (GPCR) with a seven-transmembrane domain structure. LGRs are evolutionally and structurally phylogenetic, classified into three subgroups and are members of the so-called orphan receptors. |
Product Category |
Recombinant Protein |
Target |
LGR-4 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala25-Asp951 |
Molecular Weight |
The protein has a predicted MW of 105.80 kDa. |
Activity |
Immobilized Human R-Spondin 3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human LGR-4 Nanodisc, His Tag with the EC50 of 0.36ug/ml determined by ELISA (QC Test). The affinity constant of 0.40 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in PBS (pH 7.4). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Human R Spondin 3, His Tag immobilized on CM5 Chip can bind Human LGR-4 Nanodisc, His Tag with an affinity constant of 0.40 nM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Human R-Spondin 3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human LGR-4 Nanodisc, His Tag with the EC50 of 0.36ug/ml determined by ELISA (QC Test). |
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